miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
14050 3' -46.5 NC_003521.1 + 157066 0.66 0.999998
Target:  5'- uGCUUCuuCGCcagUCGUCuuc-UCCUGg -3'
miRNA:   3'- -UGAAGuuGCGaa-AGCAGuucaAGGAC- -5'
14050 3' -46.5 NC_003521.1 + 16353 0.66 0.999997
Target:  5'- cGCUUCugcCGCUUcuacacggucaUCGUCAAGacgcaCCUGg -3'
miRNA:   3'- -UGAAGuu-GCGAA-----------AGCAGUUCaa---GGAC- -5'
14050 3' -46.5 NC_003521.1 + 37766 0.66 0.999992
Target:  5'- gGCUccCAGCGUgaUUCGUCGAGaUCCa- -3'
miRNA:   3'- -UGAa-GUUGCGa-AAGCAGUUCaAGGac -5'
14050 3' -46.5 NC_003521.1 + 181334 0.67 0.999979
Target:  5'- gGCUUCGAUgaGCUUUuugaaggCGUCGAGggUCUUGg -3'
miRNA:   3'- -UGAAGUUG--CGAAA-------GCAGUUCa-AGGAC- -5'
14050 3' -46.5 NC_003521.1 + 25075 0.69 0.99961
Target:  5'- aGCggCAACGCg-UCGUCGGGcUCCUu -3'
miRNA:   3'- -UGaaGUUGCGaaAGCAGUUCaAGGAc -5'
14050 3' -46.5 NC_003521.1 + 154332 1.11 0.029508
Target:  5'- gACUUCAACGCUUUCGUCAAGUUCCUGg -3'
miRNA:   3'- -UGAAGUUGCGAAAGCAGUUCAAGGAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.