Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
14050 | 3' | -46.5 | NC_003521.1 | + | 157066 | 0.66 | 0.999998 |
Target: 5'- uGCUUCuuCGCcagUCGUCuuc-UCCUGg -3' miRNA: 3'- -UGAAGuuGCGaa-AGCAGuucaAGGAC- -5' |
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14050 | 3' | -46.5 | NC_003521.1 | + | 16353 | 0.66 | 0.999997 |
Target: 5'- cGCUUCugcCGCUUcuacacggucaUCGUCAAGacgcaCCUGg -3' miRNA: 3'- -UGAAGuu-GCGAA-----------AGCAGUUCaa---GGAC- -5' |
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14050 | 3' | -46.5 | NC_003521.1 | + | 37766 | 0.66 | 0.999992 |
Target: 5'- gGCUccCAGCGUgaUUCGUCGAGaUCCa- -3' miRNA: 3'- -UGAa-GUUGCGa-AAGCAGUUCaAGGac -5' |
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14050 | 3' | -46.5 | NC_003521.1 | + | 181334 | 0.67 | 0.999979 |
Target: 5'- gGCUUCGAUgaGCUUUuugaaggCGUCGAGggUCUUGg -3' miRNA: 3'- -UGAAGUUG--CGAAA-------GCAGUUCa-AGGAC- -5' |
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14050 | 3' | -46.5 | NC_003521.1 | + | 25075 | 0.69 | 0.99961 |
Target: 5'- aGCggCAACGCg-UCGUCGGGcUCCUu -3' miRNA: 3'- -UGaaGUUGCGaaAGCAGUUCaAGGAc -5' |
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14050 | 3' | -46.5 | NC_003521.1 | + | 154332 | 1.11 | 0.029508 |
Target: 5'- gACUUCAACGCUUUCGUCAAGUUCCUGg -3' miRNA: 3'- -UGAAGUUGCGAAAGCAGUUCAAGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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