Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14059 | 3' | -60.1 | NC_003521.1 | + | 239387 | 0.66 | 0.867843 |
Target: 5'- gUUGCUAGGcGGCGUuCCCUGGUGu- -3' miRNA: 3'- gGACGGUCCcCCGCAuGGGGUCAUcc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 235709 | 0.68 | 0.772551 |
Target: 5'- ----aCAGGGGGCGUaGCCCgAGUAa- -3' miRNA: 3'- ggacgGUCCCCCGCA-UGGGgUCAUcc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 233443 | 0.66 | 0.867843 |
Target: 5'- --gGCCGGGcaGGGCGUAacguaCCCAGgagacGGa -3' miRNA: 3'- ggaCGGUCC--CCCGCAUg----GGGUCau---CC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 219422 | 0.71 | 0.594674 |
Target: 5'- gCUGCCAGaGGGcCGUAUCCCAGc--- -3' miRNA: 3'- gGACGGUCcCCC-GCAUGGGGUCaucc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 218548 | 0.67 | 0.806664 |
Target: 5'- gCUGCCAGGcGaGGCGUgguagACCCCGa---- -3' miRNA: 3'- gGACGGUCC-C-CCGCA-----UGGGGUcaucc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 202739 | 0.67 | 0.789856 |
Target: 5'- --cGgCAGGGGcGCGgggACCCCGuGUGGa -3' miRNA: 3'- ggaCgGUCCCC-CGCa--UGGGGU-CAUCc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 195646 | 0.67 | 0.805836 |
Target: 5'- uCCgagGCCAGGGagucGcaccaccacuuacGCGUGCgguCCCAGUAGGu -3' miRNA: 3'- -GGa--CGGUCCC----C-------------CGCAUG---GGGUCAUCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 172774 | 0.68 | 0.767272 |
Target: 5'- gCUGCCAccGGGGGCGccgcggaccacgccGCCCguGgcGGu -3' miRNA: 3'- gGACGGU--CCCCCGCa-------------UGGGguCauCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 150492 | 1.12 | 0.001419 |
Target: 5'- cCCUGCCAGGGGGCGUACCCCAGUAGGg -3' miRNA: 3'- -GGACGGUCCCCCGCAUGGGGUCAUCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 150461 | 0.69 | 0.690117 |
Target: 5'- uCCUGguCUGGGGcGGCGaGCgCCUGGUGGGg -3' miRNA: 3'- -GGAC--GGUCCC-CCGCaUG-GGGUCAUCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 135991 | 0.66 | 0.853555 |
Target: 5'- gCgUGUCGGGGGGCcgcGUcacGCCCguGUucucGGGg -3' miRNA: 3'- -GgACGGUCCCCCG---CA---UGGGguCA----UCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 129836 | 0.69 | 0.7275 |
Target: 5'- gCUGCUGGGGcccguGGcCGUGCCCUgcuacugcgacgAGUGGGu -3' miRNA: 3'- gGACGGUCCC-----CC-GCAUGGGG------------UCAUCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 124190 | 0.66 | 0.838565 |
Target: 5'- gCCUGCCGgcuGGGGGUGagcggGCCCUuucuGGc -3' miRNA: 3'- -GGACGGU---CCCCCGCa----UGGGGucauCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 119799 | 0.69 | 0.690117 |
Target: 5'- uCCggcGCCAGcGGGGCGacacggUggugcgcacGCCCCAGUGGu -3' miRNA: 3'- -GGa--CGGUC-CCCCGC------A---------UGGGGUCAUCc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 117496 | 0.71 | 0.575675 |
Target: 5'- gCCUGuCCGGcGGGGCGUacggcgguaugcACUCC-GUGGGc -3' miRNA: 3'- -GGAC-GGUC-CCCCGCA------------UGGGGuCAUCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 113341 | 0.67 | 0.814863 |
Target: 5'- --cGCCGGGGGGC---UUCCAGgGGGa -3' miRNA: 3'- ggaCGGUCCCCCGcauGGGGUCaUCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 112699 | 0.68 | 0.781262 |
Target: 5'- uCCUGCU-GGGGGUGgucaacGCCCUAG-AGa -3' miRNA: 3'- -GGACGGuCCCCCGCa-----UGGGGUCaUCc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 101813 | 0.68 | 0.754807 |
Target: 5'- --aGcCCAGGGGGCGgu-CgCGGUAGGc -3' miRNA: 3'- ggaC-GGUCCCCCGCaugGgGUCAUCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 92080 | 0.73 | 0.483557 |
Target: 5'- aCCggggGUCGGGGGGUgcgcagggcgGUGCCCCGGgucGGa -3' miRNA: 3'- -GGa---CGGUCCCCCG----------CAUGGGGUCau-CC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 90288 | 0.75 | 0.36058 |
Target: 5'- --cGCCcccguAGGGGGgGUACCCCGGUAa- -3' miRNA: 3'- ggaCGG-----UCCCCCgCAUGGGGUCAUcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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