Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14060 | 5' | -57.5 | NC_003521.1 | + | 4 | 0.66 | 0.895543 |
Target: 5'- aUCCGGccuccgcUGCGgguCCcGGGGGGUGGg- -3' miRNA: 3'- aAGGCC-------AUGUau-GGaUCCCCCGCCau -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 240553 | 0.66 | 0.895543 |
Target: 5'- aUCCGGccuccgcUGCGgguCCcGGGGGGUGGg- -3' miRNA: 3'- aAGGCC-------AUGUau-GGaUCCCCCGCCau -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 40326 | 0.66 | 0.895543 |
Target: 5'- aUCCGGccuccgcUGCGgguCCcGGGGGGUGGg- -3' miRNA: 3'- aAGGCC-------AUGUau-GGaUCCCCCGCCau -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 112599 | 0.66 | 0.892309 |
Target: 5'- aUCUGGUACuaucGCCUcaagAGGGGGCucuacacccagcccaGGUGg -3' miRNA: 3'- aAGGCCAUGua--UGGA----UCCCCCG---------------CCAU- -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 97843 | 0.66 | 0.889682 |
Target: 5'- -gCCGGUGCG-ACagagAGGGGuGCGGg- -3' miRNA: 3'- aaGGCCAUGUaUGga--UCCCC-CGCCau -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 25298 | 0.67 | 0.846286 |
Target: 5'- -aCCGuGUAC--ACCUAcGGGGCGGUc -3' miRNA: 3'- aaGGC-CAUGuaUGGAUcCCCCGCCAu -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 5708 | 0.68 | 0.83837 |
Target: 5'- -cCCGGggGCugucuauCgCUAGGGGGUGGUAg -3' miRNA: 3'- aaGGCCa-UGuau----G-GAUCCCCCGCCAU- -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 150439 | 0.68 | 0.830275 |
Target: 5'- aUUCUagGGUACGUACCUAGGuuaGGGUcGUAu -3' miRNA: 3'- -AAGG--CCAUGUAUGGAUCC---CCCGcCAU- -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 150165 | 0.68 | 0.821172 |
Target: 5'- aUCCGGgGCGUGuccaCUAGGGGGUguaccuaGGUAc -3' miRNA: 3'- aAGGCCaUGUAUg---GAUCCCCCG-------CCAU- -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 98731 | 0.68 | 0.813576 |
Target: 5'- ---gGGUACcUGCCgcGGGGGUGGUc -3' miRNA: 3'- aaggCCAUGuAUGGauCCCCCGCCAu -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 90621 | 0.7 | 0.721947 |
Target: 5'- -aCCGGaUACgGUACCccGGaGGGGCGGUAc -3' miRNA: 3'- aaGGCC-AUG-UAUGGa-UC-CCCCGCCAU- -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 150306 | 0.71 | 0.652911 |
Target: 5'- --gUGG-GCGUACCUAGGGGGCGuGa- -3' miRNA: 3'- aagGCCaUGUAUGGAUCCCCCGC-Cau -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 150473 | 0.72 | 0.592997 |
Target: 5'- gUUCCGGUACGguguauagcccUGCC-AGGGGGCGu-- -3' miRNA: 3'- -AAGGCCAUGU-----------AUGGaUCCCCCGCcau -5' |
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14060 | 5' | -57.5 | NC_003521.1 | + | 150247 | 1.04 | 0.006366 |
Target: 5'- cUUCCGGUACAUACCUAGGGGGCGGUAc -3' miRNA: 3'- -AAGGCCAUGUAUGGAUCCCCCGCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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