Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14066 | 5' | -63.2 | NC_003521.1 | + | 29411 | 0.66 | 0.707814 |
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Target: 5'- gAGGacagucCGGGCCCCGGUGAcaGCUAcaaccaGCGc -3' miRNA: 3'- -UCC------GCCCGGGGCCGCU--CGAUaca---CGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 224745 | 0.66 | 0.716155 |
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Target: 5'- cAGGCggccagcgcgucgGGGCUCCGGC-AGU--UGUGCa -3' miRNA: 3'- -UCCG-------------CCCGGGGCCGcUCGauACACGc -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 212710 | 0.66 | 0.716155 |
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Target: 5'- uGGGuCGGGCCUCgaucuugGGCGAGCgcc-UGCu -3' miRNA: 3'- -UCC-GCCCGGGG-------CCGCUCGauacACGc -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 131056 | 0.66 | 0.717079 |
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Target: 5'- cGGCGGGCCuCUGGac-GCgGUGgcgGCGa -3' miRNA: 3'- uCCGCCCGG-GGCCgcuCGaUACa--CGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 148428 | 0.66 | 0.717079 |
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Target: 5'- cGGGCGGccGCgCCGGCGgcuccagggguGGCcg-GUGCGu -3' miRNA: 3'- -UCCGCC--CGgGGCCGC-----------UCGauaCACGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 19340 | 0.66 | 0.717079 |
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Target: 5'- cGG-GGaGCUCCGGCGAGgaGcccucGUGCGa -3' miRNA: 3'- uCCgCC-CGGGGCCGCUCgaUa----CACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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