Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14066 | 5' | -63.2 | NC_003521.1 | + | 177736 | 0.72 | 0.354315 |
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Target: 5'- aGGGCGGGCagcgcgCCGGCGcAGCUGUacgggGUcGCGg -3' miRNA: 3'- -UCCGCCCGg-----GGCCGC-UCGAUA-----CA-CGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 210025 | 0.72 | 0.339923 |
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Target: 5'- cGGCGGGCCCuCGGUGcGCUAcc-GCGc -3' miRNA: 3'- uCCGCCCGGG-GCCGCuCGAUacaCGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 238458 | 0.73 | 0.312428 |
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Target: 5'- aGGGC-GGCCUCGGCGGGCgg-G-GCGg -3' miRNA: 3'- -UCCGcCCGGGGCCGCUCGauaCaCGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 38230 | 0.73 | 0.312428 |
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Target: 5'- aGGGC-GGCCUCGGCGGGCgg-G-GCGg -3' miRNA: 3'- -UCCGcCCGGGGCCGCUCGauaCaCGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 87743 | 0.74 | 0.274407 |
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Target: 5'- uGGUGGGCCCCGGCccGGGCcccg-GCGg -3' miRNA: 3'- uCCGCCCGGGGCCG--CUCGauacaCGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 146795 | 1.07 | 0.001406 |
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Target: 5'- aAGGCGGGCCCCGGCGAGCUAUGUGCGu -3' miRNA: 3'- -UCCGCCCGGGGCCGCUCGAUACACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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