Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14066 | 5' | -63.2 | NC_003521.1 | + | 39890 | 0.7 | 0.483952 |
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Target: 5'- gGGGUGGGCgugcggucguCCCGGCGGGCgcgaaaGCGc -3' miRNA: 3'- -UCCGCCCG----------GGGCCGCUCGauaca-CGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 155332 | 0.7 | 0.483952 |
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Target: 5'- -cGCGGGUCCCGGCGGGg---GcGCGg -3' miRNA: 3'- ucCGCCCGGGGCCGCUCgauaCaCGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 198584 | 0.7 | 0.483952 |
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Target: 5'- cGGCGGGCUagaCGcCGAGCUGagguugugGUGCGa -3' miRNA: 3'- uCCGCCCGGg--GCcGCUCGAUa-------CACGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 187252 | 0.69 | 0.492816 |
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Target: 5'- gAGGC-GGCCCCGGgaGAGCUAgagagacGCGa -3' miRNA: 3'- -UCCGcCCGGGGCCg-CUCGAUaca----CGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 178800 | 0.69 | 0.510763 |
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Target: 5'- cGGGCGaGGCaugCCCGGCGGGCgg-GU-CGa -3' miRNA: 3'- -UCCGC-CCG---GGGCCGCUCGauaCAcGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 146795 | 1.07 | 0.001406 |
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Target: 5'- aAGGCGGGCCCCGGCGAGCUAUGUGCGu -3' miRNA: 3'- -UCCGCCCGGGGCCGCUCGAUACACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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