Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14066 | 5' | -63.2 | NC_003521.1 | + | 113584 | 0.66 | 0.679715 |
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Target: 5'- gGGGCuGGGCCUCGG-GGGCaacgGggGCGg -3' miRNA: 3'- -UCCG-CCCGGGGCCgCUCGaua-Ca-CGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 126777 | 0.66 | 0.669326 |
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Target: 5'- cGGCaGGCCCUGGCGcugcgcgugguccAGCUccGcGCGc -3' miRNA: 3'- uCCGcCCGGGGCCGC-------------UCGAuaCaCGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 77730 | 0.67 | 0.660802 |
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Target: 5'- cGGC-GGCCUCGGUGGGCg----GCGu -3' miRNA: 3'- uCCGcCCGGGGCCGCUCGauacaCGC- -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 224019 | 0.67 | 0.651312 |
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Target: 5'- cGGCGGGCCCggcggucggGGCGGGCgccGUcGCc -3' miRNA: 3'- uCCGCCCGGGg--------CCGCUCGauaCA-CGc -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 150662 | 0.67 | 0.640858 |
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Target: 5'- aAGGCGGcuCUCCGGCGGcggcgccgcugcuGCUggGUGCu -3' miRNA: 3'- -UCCGCCc-GGGGCCGCU-------------CGAuaCACGc -5' |
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| 14066 | 5' | -63.2 | NC_003521.1 | + | 238458 | 0.73 | 0.312428 |
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Target: 5'- aGGGC-GGCCUCGGCGGGCgg-G-GCGg -3' miRNA: 3'- -UCCGcCCGGGGCCGCUCGauaCaCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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