Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1407 | 5' | -55.7 | NC_001335.1 | + | 4278 | 0.66 | 0.618539 |
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Target: 5'- gUCGGGCgCUCUGCCauUGAGCU--ACAc -3' miRNA: 3'- aAGCUCG-GAGACGG--ACUUGGacUGUu -5' |
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| 1407 | 5' | -55.7 | NC_001335.1 | + | 3233 | 0.66 | 0.607198 |
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Target: 5'- cUCGAGaugCUCUGCCUGGucacagGCCggGACu- -3' miRNA: 3'- aAGCUCg--GAGACGGACU------UGGa-CUGuu -5' |
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| 1407 | 5' | -55.7 | NC_001335.1 | + | 32960 | 0.67 | 0.551024 |
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Target: 5'- -cCGAGCCggccccggCU-CCUGAGCCUGAgAc -3' miRNA: 3'- aaGCUCGGa-------GAcGGACUUGGACUgUu -5' |
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| 1407 | 5' | -55.7 | NC_001335.1 | + | 29901 | 0.67 | 0.507253 |
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Target: 5'- -gCGAGggUCUGCaaGAGCCUGACAc -3' miRNA: 3'- aaGCUCggAGACGgaCUUGGACUGUu -5' |
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| 1407 | 5' | -55.7 | NC_001335.1 | + | 5894 | 0.68 | 0.496537 |
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Target: 5'- cUCGGGCCuggUCUGCCUGccgauACCgcacggGACGu -3' miRNA: 3'- aAGCUCGG---AGACGGACu----UGGa-----CUGUu -5' |
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| 1407 | 5' | -55.7 | NC_001335.1 | + | 11012 | 0.68 | 0.496536 |
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Target: 5'- cUCGAGCCUCUGCCgcu-UCUacGGCGu -3' miRNA: 3'- aAGCUCGGAGACGGacuuGGA--CUGUu -5' |
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| 1407 | 5' | -55.7 | NC_001335.1 | + | 49866 | 0.68 | 0.485923 |
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Target: 5'- gUCGAGCCUCUGCgaGuGCagguaGACAGc -3' miRNA: 3'- aAGCUCGGAGACGgaCuUGga---CUGUU- -5' |
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| 1407 | 5' | -55.7 | NC_001335.1 | + | 34924 | 0.69 | 0.424705 |
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Target: 5'- cUUCGAGCgUCagGCUUGGuCCUGGCGu -3' miRNA: 3'- -AAGCUCGgAGa-CGGACUuGGACUGUu -5' |
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| 1407 | 5' | -55.7 | NC_001335.1 | + | 43051 | 1.04 | 0.001384 |
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Target: 5'- cUUCGAGCCUCUGCCUGAACCUGACAAa -3' miRNA: 3'- -AAGCUCGGAGACGGACUUGGACUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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