Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1408 | 3' | -53.8 | NC_001335.1 | + | 40107 | 0.67 | 0.70979 |
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Target: 5'- gCGAGGCuugCGGGGAUc-CGGgcUCUCCg -3' miRNA: 3'- -GCUCCGua-GCUCCUAcuGCU--AGAGGa -5' |
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| 1408 | 3' | -53.8 | NC_001335.1 | + | 24328 | 0.68 | 0.687958 |
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Target: 5'- aCGAGGCuAUCGcuGA-GGCGAUgUCCa -3' miRNA: 3'- -GCUCCG-UAGCucCUaCUGCUAgAGGa -5' |
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| 1408 | 3' | -53.8 | NC_001335.1 | + | 33848 | 0.68 | 0.665907 |
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Target: 5'- cCGGGcGCAUCGucuGAUGACGAgauUCCUg -3' miRNA: 3'- -GCUC-CGUAGCuc-CUACUGCUag-AGGA- -5' |
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| 1408 | 3' | -53.8 | NC_001335.1 | + | 29147 | 0.69 | 0.632615 |
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Target: 5'- uGAGGCAgUGGGGAUcuCGAUC-CCUg -3' miRNA: 3'- gCUCCGUaGCUCCUAcuGCUAGaGGA- -5' |
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| 1408 | 3' | -53.8 | NC_001335.1 | + | 15205 | 0.7 | 0.555358 |
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Target: 5'- uCGAGGU--CGGGGAUGuccGCGAaCUCCUu -3' miRNA: 3'- -GCUCCGuaGCUCCUAC---UGCUaGAGGA- -5' |
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| 1408 | 3' | -53.8 | NC_001335.1 | + | 33208 | 0.71 | 0.523012 |
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Target: 5'- aCGAGGC--CGAGGAUGucgcagcCGAUCUCa- -3' miRNA: 3'- -GCUCCGuaGCUCCUACu------GCUAGAGga -5' |
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| 1408 | 3' | -53.8 | NC_001335.1 | + | 44458 | 1.09 | 0.001467 |
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Target: 5'- cCGAGGCAUCGAGGAUGACGAUCUCCUg -3' miRNA: 3'- -GCUCCGUAGCUCCUACUGCUAGAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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