Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1409 | 5' | -49 | NC_001335.1 | + | 14233 | 0.66 | 0.969488 |
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Target: 5'- uGGUa--GaGGGAGCugAACUGAGUGg- -3' miRNA: 3'- -CCGcagC-CCCUUGugUUGACUUAUac -5' |
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| 1409 | 5' | -49 | NC_001335.1 | + | 26438 | 0.66 | 0.962308 |
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Target: 5'- cGGCaUCGGaGGAgGCGCGACcGggUAc- -3' miRNA: 3'- -CCGcAGCC-CCU-UGUGUUGaCuuAUac -5' |
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| 1409 | 5' | -49 | NC_001335.1 | + | 21210 | 0.66 | 0.958309 |
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Target: 5'- uGGCGUCGGGGcgaucACGCAuCcGggUu-- -3' miRNA: 3'- -CCGCAGCCCCu----UGUGUuGaCuuAuac -5' |
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| 1409 | 5' | -49 | NC_001335.1 | + | 34832 | 0.67 | 0.948035 |
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Target: 5'- gGGCcUCGGGGGucaagaacGCGCAgcaccucuucaacuGCUGggUAg- -3' miRNA: 3'- -CCGcAGCCCCU--------UGUGU--------------UGACuuAUac -5' |
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| 1409 | 5' | -49 | NC_001335.1 | + | 16379 | 0.67 | 0.933994 |
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Target: 5'- aGCGUCGGGGcAGCgucgagGCGGCUGccuugAUGc -3' miRNA: 3'- cCGCAGCCCC-UUG------UGUUGACuua--UAC- -5' |
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| 1409 | 5' | -49 | NC_001335.1 | + | 45013 | 1.13 | 0.002406 |
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Target: 5'- cGGCGUCGGGGAACACAACUGAAUAUGg -3' miRNA: 3'- -CCGCAGCCCCUUGUGUUGACUUAUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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