Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14092 | 5' | -54.8 | NC_003521.1 | + | 15754 | 0.68 | 0.935407 |
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Target: 5'- gCGCCUggaaUGGUucugcaaaacGUCGUUCUACGcgGCg -3' miRNA: 3'- -GCGGAa---ACCG----------UAGCAGGGUGCuaCGa -5' |
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| 14092 | 5' | -54.8 | NC_003521.1 | + | 62649 | 0.68 | 0.935407 |
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Target: 5'- cCGUCUcguaGGUAucugcguguUCGUCCUGCGGUGCUu -3' miRNA: 3'- -GCGGAaa--CCGU---------AGCAGGGUGCUACGA- -5' |
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| 14092 | 5' | -54.8 | NC_003521.1 | + | 223620 | 0.68 | 0.925285 |
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Target: 5'- gGCCUccgucUGGUAggCGU-CCACGAUGCg -3' miRNA: 3'- gCGGAa----ACCGUa-GCAgGGUGCUACGa -5' |
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| 14092 | 5' | -54.8 | NC_003521.1 | + | 235167 | 0.69 | 0.90718 |
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Target: 5'- gCGCgaUUGGCGUCGcgucaugguggaaUCCCACGcccugugGUGCg -3' miRNA: 3'- -GCGgaAACCGUAGC-------------AGGGUGC-------UACGa -5' |
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| 14092 | 5' | -54.8 | NC_003521.1 | + | 194752 | 0.7 | 0.868534 |
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Target: 5'- aCGCCg--GGCGUCGcgaccucaUCCCGCccGGUGCc -3' miRNA: 3'- -GCGGaaaCCGUAGC--------AGGGUG--CUACGa -5' |
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| 14092 | 5' | -54.8 | NC_003521.1 | + | 178601 | 0.7 | 0.86115 |
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Target: 5'- aGCCgaagaccUUGGCGUCGUCCgUGCGGauuUGCa -3' miRNA: 3'- gCGGa------AACCGUAGCAGG-GUGCU---ACGa -5' |
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| 14092 | 5' | -54.8 | NC_003521.1 | + | 149253 | 0.71 | 0.829689 |
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Target: 5'- aCGCCUacgacgUGGCcauGUCGUUCCGCGucgGCg -3' miRNA: 3'- -GCGGAa-----ACCG---UAGCAGGGUGCua-CGa -5' |
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| 14092 | 5' | -54.8 | NC_003521.1 | + | 233366 | 0.71 | 0.821374 |
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Target: 5'- gGCUUUUcGCA-CGUCCCGCGugGUGCUg -3' miRNA: 3'- gCGGAAAcCGUaGCAGGGUGC--UACGA- -5' |
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| 14092 | 5' | -54.8 | NC_003521.1 | + | 134208 | 1.07 | 0.00771 |
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Target: 5'- uCGCCUUUGGCAUCGUCCCACGAUGCUc -3' miRNA: 3'- -GCGGAAACCGUAGCAGGGUGCUACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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