Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14111 | 3' | -51.9 | NC_003521.1 | + | 123817 | 0.78 | 0.645497 |
Target: 5'- cUCGUUGCgGUCGUcuucgUCCUCCUCc-- -3' miRNA: 3'- uAGCAACGgCAGCAa----AGGAGGAGauc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 93947 | 0.8 | 0.523924 |
Target: 5'- -----cGCCGUCGUcuggcUUCCUCCUCUGGg -3' miRNA: 3'- uagcaaCGGCAGCA-----AAGGAGGAGAUC- -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 197134 | 0.84 | 0.35402 |
Target: 5'- gGUCGUUGUCGUCGUcUCCUCCUgCUGc -3' miRNA: 3'- -UAGCAACGGCAGCAaAGGAGGA-GAUc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 124562 | 1.08 | 0.012592 |
Target: 5'- cAUCGUUGCCGUCGUUUCCUCCUCUAGg -3' miRNA: 3'- -UAGCAACGGCAGCAAAGGAGGAGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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