Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1414 | 5' | -58.7 | NC_001335.1 | + | 6717 | 0.66 | 0.432918 |
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Target: 5'- uGAGgGGGAGAcGggGCaaGCUCCGGguGg -3' miRNA: 3'- -UUCgCUCUCUaCuuCG--CGGGGCCguU- -5' |
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| 1414 | 5' | -58.7 | NC_001335.1 | + | 22373 | 0.66 | 0.429044 |
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Target: 5'- uGAGCGAuGAGAUGGAGUggcugcagaccgcaGCCucgaaugcagcuaCCGGCAu -3' miRNA: 3'- -UUCGCU-CUCUACUUCG--------------CGG-------------GGCCGUu -5' |
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| 1414 | 5' | -58.7 | NC_001335.1 | + | 17586 | 0.66 | 0.423272 |
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Target: 5'- uGGCGAGAGccGAcuGGaucauCCCCGGCAGu -3' miRNA: 3'- uUCGCUCUCuaCU--UCgc---GGGGCCGUU- -5' |
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| 1414 | 5' | -58.7 | NC_001335.1 | + | 28677 | 0.66 | 0.423272 |
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Target: 5'- -cGCGccaaGGAGAUGGAGCGCCgaGuGCGc -3' miRNA: 3'- uuCGC----UCUCUACUUCGCGGggC-CGUu -5' |
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| 1414 | 5' | -58.7 | NC_001335.1 | + | 34897 | 0.66 | 0.417546 |
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Target: 5'- aGAGCGAGAagaucucguucgucuGAUGAccaaguucaaGGUGCUgCCGGCAGg -3' miRNA: 3'- -UUCGCUCU---------------CUACU----------UCGCGG-GGCCGUU- -5' |
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| 1414 | 5' | -58.7 | NC_001335.1 | + | 23276 | 0.66 | 0.413755 |
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Target: 5'- cGGGUGAccGAGuucGGAGUGCCCUGGCc- -3' miRNA: 3'- -UUCGCU--CUCua-CUUCGCGGGGCCGuu -5' |
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| 1414 | 5' | -58.7 | NC_001335.1 | + | 29117 | 0.66 | 0.410925 |
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Target: 5'- cAAGCGAGuucAUGAGGCGuucucugcgacauaCCCCGGUg- -3' miRNA: 3'- -UUCGCUCuc-UACUUCGC--------------GGGGCCGuu -5' |
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| 1414 | 5' | -58.7 | NC_001335.1 | + | 48798 | 0.68 | 0.350137 |
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Target: 5'- cGGUGAGAGcgGAuacaucaAGCGCaaCGGCAAg -3' miRNA: 3'- uUCGCUCUCuaCU-------UCGCGggGCCGUU- -5' |
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| 1414 | 5' | -58.7 | NC_001335.1 | + | 47116 | 1.04 | 0.00074 |
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Target: 5'- cAAGCGAGAGAUGAAGCGCCCCGGCAAg -3' miRNA: 3'- -UUCGCUCUCUACUUCGCGGGGCCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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