Results 1 - 20 of 62 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14146 | 3' | -52.5 | NC_003521.1 | + | 124287 | 0.66 | 0.996643 |
Target: 5'- cUGAcGAUCUCCaGAAagCGCgGCGaGGUg -3' miRNA: 3'- aGCUuUUAGAGG-CUUa-GCGgCGC-CCA- -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 148843 | 0.66 | 0.996643 |
Target: 5'- cCGu---UCgcgCCGGAUCgGCCGcCGGGUu -3' miRNA: 3'- aGCuuuuAGa--GGCUUAG-CGGC-GCCCA- -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 85370 | 0.66 | 0.996643 |
Target: 5'- cUCGAcgGgcugCUgCGAcUCGCCcaGCGGGUc -3' miRNA: 3'- -AGCUuuUa---GAgGCUuAGCGG--CGCCCA- -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 119373 | 0.66 | 0.996643 |
Target: 5'- cCGggGAUgUCCGccgggaagaaGAUcaCGUCGUGGGUg -3' miRNA: 3'- aGCuuUUAgAGGC----------UUA--GCGGCGCCCA- -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 45276 | 0.66 | 0.996643 |
Target: 5'- cUGGAGAUCauggaCCGGG-CGCCGCuGGGc -3' miRNA: 3'- aGCUUUUAGa----GGCUUaGCGGCG-CCCa -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 177519 | 0.66 | 0.996643 |
Target: 5'- gCGGAGAUCUCUcauacacaGAAgCGCCGCGa-- -3' miRNA: 3'- aGCUUUUAGAGG--------CUUaGCGGCGCcca -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 13860 | 0.66 | 0.996482 |
Target: 5'- gCGggGGUCUCUGcgggcGGcgccgccauggauuUCGCCGgGGGg -3' miRNA: 3'- aGCuuUUAGAGGC-----UU--------------AGCGGCgCCCa -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 202988 | 0.66 | 0.996083 |
Target: 5'- uUCGAucuacAGUCUCCGGGcggcUCGCCcgccaagaCGGGUu -3' miRNA: 3'- -AGCUu----UUAGAGGCUU----AGCGGc-------GCCCA- -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 193287 | 0.66 | 0.996083 |
Target: 5'- cCGGGAcgggCgUCgGAGUCGgCGCGGGg -3' miRNA: 3'- aGCUUUua--G-AGgCUUAGCgGCGCCCa -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 189546 | 0.66 | 0.996023 |
Target: 5'- cCGAGAAaC-CCGAGUUGCugaucgaCGUGGGUa -3' miRNA: 3'- aGCUUUUaGaGGCUUAGCG-------GCGCCCA- -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 46432 | 0.66 | 0.995449 |
Target: 5'- gCGAGGGUaUCCGGcgggGCUGCGGGg -3' miRNA: 3'- aGCUUUUAgAGGCUuag-CGGCGCCCa -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 135362 | 0.66 | 0.995449 |
Target: 5'- gCGcc-GUCUCCGcggcgguuAUCGCCcGCGGGc -3' miRNA: 3'- aGCuuuUAGAGGCu-------UAGCGG-CGCCCa -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 43796 | 0.66 | 0.995381 |
Target: 5'- uUCGAAAggggggccgccauGUCgcagcagCCGggUCagccgccgccgGCCGCGGGc -3' miRNA: 3'- -AGCUUU-------------UAGa------GGCuuAG-----------CGGCGCCCa -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 223939 | 0.66 | 0.994733 |
Target: 5'- cCGAGGGgg-UCGAGgcgGCCGCGGGg -3' miRNA: 3'- aGCUUUUagaGGCUUag-CGGCGCCCa -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 143304 | 0.66 | 0.994733 |
Target: 5'- gUCGucucUCUCCGuGGUCGCCcGCGGu- -3' miRNA: 3'- -AGCuuuuAGAGGC-UUAGCGG-CGCCca -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 95133 | 0.66 | 0.994262 |
Target: 5'- cUUGAAGAgCUCCGcGUCGUgcgagagcaggaacuCGCGGGc -3' miRNA: 3'- -AGCUUUUaGAGGCuUAGCG---------------GCGCCCa -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 133484 | 0.66 | 0.993929 |
Target: 5'- gUCGAGGAUCaccuggcgCCGc--UGCUGCGGGg -3' miRNA: 3'- -AGCUUUUAGa-------GGCuuaGCGGCGCCCa -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 123669 | 0.66 | 0.993929 |
Target: 5'- cUUGGAGGgagCCGGcgCGCUGCGGcGUg -3' miRNA: 3'- -AGCUUUUagaGGCUuaGCGGCGCC-CA- -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 125588 | 0.66 | 0.993929 |
Target: 5'- cUCGAcGcagauguacugcGUgUCCGuGUCGCCGCGGa- -3' miRNA: 3'- -AGCUuU------------UAgAGGCuUAGCGGCGCCca -5' |
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14146 | 3' | -52.5 | NC_003521.1 | + | 104957 | 0.67 | 0.993029 |
Target: 5'- cUCGAAGGUCgugCCGcgggggCGCCGCGa-- -3' miRNA: 3'- -AGCUUUUAGa--GGCuua---GCGGCGCcca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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