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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 14179 | 3' | -51.9 | NC_003521.1 | + | 220415 | 0.71 | 0.914424 |
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Target: 5'- cGAAGGUgaagGCCAGCUGaaagacGAGCACGg -3' miRNA: 3'- uCUUCUAga--CGGUCGACga----CUUGUGC- -5' |
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| 14179 | 3' | -51.9 | NC_003521.1 | + | 193159 | 0.71 | 0.908386 |
|
Target: 5'- cGGggGcGUCUGCU-GCUGCUGuuGCGCa -3' miRNA: 3'- -UCuuC-UAGACGGuCGACGACu-UGUGc -5' |
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| 14179 | 3' | -51.9 | NC_003521.1 | + | 75259 | 0.71 | 0.931077 |
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Target: 5'- cGAGGAgg-GCCGGCUGCacauGCACGu -3' miRNA: 3'- uCUUCUagaCGGUCGACGacu-UGUGC- -5' |
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| 14179 | 3' | -51.9 | NC_003521.1 | + | 167359 | 0.72 | 0.874646 |
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Target: 5'- aAGAcGAUCga-CAGCUGCUGggUACa -3' miRNA: 3'- -UCUuCUAGacgGUCGACGACuuGUGc -5' |
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| 14179 | 3' | -51.9 | NC_003521.1 | + | 8035 | 0.73 | 0.85171 |
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Target: 5'- cGGAGGcgUUGagaaCCAGCUGCUGAGgGCa -3' miRNA: 3'- -UCUUCuaGAC----GGUCGACGACUUgUGc -5' |
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| 14179 | 3' | -51.9 | NC_003521.1 | + | 67740 | 0.76 | 0.713975 |
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Target: 5'- -cAAGAUCuucucgcaacagUGCCGGCUGCUGAACGa- -3' miRNA: 3'- ucUUCUAG------------ACGGUCGACGACUUGUgc -5' |
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| 14179 | 3' | -51.9 | NC_003521.1 | + | 87475 | 1.09 | 0.009872 |
|
Target: 5'- cAGAAGAUCUGCCAGCUGCUGAACACGu -3' miRNA: 3'- -UCUUCUAGACGGUCGACGACUUGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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