Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14179 | 3' | -51.9 | NC_003521.1 | + | 196443 | 0.69 | 0.968103 |
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Target: 5'- --cGGGUCgGCCGGCUGCc--GCGCGa -3' miRNA: 3'- ucuUCUAGaCGGUCGACGacuUGUGC- -5' |
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| 14179 | 3' | -51.9 | NC_003521.1 | + | 198742 | 0.66 | 0.993029 |
|
Target: 5'- cAGAcgAGGUCuUGCCGuGCUcGCUGGuCGCGc -3' miRNA: 3'- -UCU--UCUAG-ACGGU-CGA-CGACUuGUGC- -5' |
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| 14179 | 3' | -51.9 | NC_003521.1 | + | 199577 | 0.7 | 0.949876 |
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Target: 5'- cGAGGccacguucGUCcccagcagUGCUGGUUGCUGAGCACGa -3' miRNA: 3'- uCUUC--------UAG--------ACGGUCGACGACUUGUGC- -5' |
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| 14179 | 3' | -51.9 | NC_003521.1 | + | 206113 | 0.69 | 0.961488 |
|
Target: 5'- aGGGAGGcCgGUCAGCUGCU--GCGCGa -3' miRNA: 3'- -UCUUCUaGaCGGUCGACGAcuUGUGC- -5' |
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| 14179 | 3' | -51.9 | NC_003521.1 | + | 211224 | 0.66 | 0.996141 |
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Target: 5'- uGAAGGUCUugGUCAGCagGCUGuugggaauACACa -3' miRNA: 3'- uCUUCUAGA--CGGUCGa-CGACu-------UGUGc -5' |
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| 14179 | 3' | -51.9 | NC_003521.1 | + | 213769 | 0.66 | 0.995498 |
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Target: 5'- uAGAAGAUg-GCCAGC-GCguaGACGCGc -3' miRNA: 3'- -UCUUCUAgaCGGUCGaCGac-UUGUGC- -5' |
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| 14179 | 3' | -51.9 | NC_003521.1 | + | 220415 | 0.71 | 0.914424 |
|
Target: 5'- cGAAGGUgaagGCCAGCUGaaagacGAGCACGg -3' miRNA: 3'- uCUUCUAga--CGGUCGACga----CUUGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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