Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1418 | 3' | -54.3 | NC_001335.1 | + | 583 | 0.72 | 0.413263 |
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Target: 5'- --aCG-CCGcCAAGAACGGCGGCGcGCu -3' miRNA: 3'- guaGCaGGC-GUUCUUGCUGUCGCcCG- -5' |
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| 1418 | 3' | -54.3 | NC_001335.1 | + | 34316 | 0.74 | 0.31039 |
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Target: 5'- ----uUCUGCAGcaucacagcGAACGGCAGCGGGCc -3' miRNA: 3'- guagcAGGCGUU---------CUUGCUGUCGCCCG- -5' |
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| 1418 | 3' | -54.3 | NC_001335.1 | + | 24114 | 0.75 | 0.287917 |
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Target: 5'- gGUC-UCCGCAcAGAGCGGCGGUGcuGGCg -3' miRNA: 3'- gUAGcAGGCGU-UCUUGCUGUCGC--CCG- -5' |
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| 1418 | 3' | -54.3 | NC_001335.1 | + | 45399 | 0.75 | 0.280716 |
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Target: 5'- aGUCGUCCaacucauccuGCucGAACGGC-GCGGGCu -3' miRNA: 3'- gUAGCAGG----------CGuuCUUGCUGuCGCCCG- -5' |
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| 1418 | 3' | -54.3 | NC_001335.1 | + | 42094 | 0.75 | 0.266744 |
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Target: 5'- gGUCGUCgGCAAGGACGACA-CGGu- -3' miRNA: 3'- gUAGCAGgCGUUCUUGCUGUcGCCcg -5' |
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| 1418 | 3' | -54.3 | NC_001335.1 | + | 26409 | 0.77 | 0.210753 |
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Target: 5'- -uUCGUCCuGaCAGGcAUGACAGUGGGCu -3' miRNA: 3'- guAGCAGG-C-GUUCuUGCUGUCGCCCG- -5' |
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| 1418 | 3' | -54.3 | NC_001335.1 | + | 26258 | 0.78 | 0.174477 |
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Target: 5'- gGUCGggCGCAGucaAGCGGCAGCGGGCu -3' miRNA: 3'- gUAGCagGCGUUc--UUGCUGUCGCCCG- -5' |
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| 1418 | 3' | -54.3 | NC_001335.1 | + | 48922 | 1.11 | 0.000906 |
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Target: 5'- uCAUCGUCCGCAAGAACGACAGCGGGCu -3' miRNA: 3'- -GUAGCAGGCGUUCUUGCUGUCGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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