Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14182 | 5' | -58.4 | NC_003521.1 | + | 59049 | 0.66 | 0.901247 |
Target: 5'- uCGCGACUaCCGggcgcugugggCCuuCUCCCUGGUGAu -3' miRNA: 3'- -GCGCUGA-GGCa----------GGuuGGGGGGUCAUU- -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 17146 | 0.66 | 0.901247 |
Target: 5'- gCGCG-CUUCGcCCGACCCuCCCuGg-- -3' miRNA: 3'- -GCGCuGAGGCaGGUUGGG-GGGuCauu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 180122 | 0.66 | 0.888638 |
Target: 5'- uGCGAC-CCGcgCC-GCCCCCgGGUc- -3' miRNA: 3'- gCGCUGaGGCa-GGuUGGGGGgUCAuu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 29552 | 0.66 | 0.888638 |
Target: 5'- gCGCGACccaCCcaCCGACacaCCCCGGUGGa -3' miRNA: 3'- -GCGCUGa--GGcaGGUUGg--GGGGUCAUU- -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 230609 | 0.66 | 0.882021 |
Target: 5'- -uCGugUCUGUCCuuCCCCCCuccccGUGu -3' miRNA: 3'- gcGCugAGGCAGGuuGGGGGGu----CAUu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 95744 | 0.66 | 0.874507 |
Target: 5'- gGCGGCUgagccacagguagUCGUCCGAgCCgCCCAGa-- -3' miRNA: 3'- gCGCUGA-------------GGCAGGUUgGG-GGGUCauu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 129861 | 0.66 | 0.873115 |
Target: 5'- gGCGAC-CCGUCCGcuuCCUccgcgagaacaacaCCCAGUGc -3' miRNA: 3'- gCGCUGaGGCAGGUu--GGG--------------GGGUCAUu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 68427 | 0.67 | 0.860965 |
Target: 5'- gCGaCGGCUCCG-CCGgcGCCCCUCAa--- -3' miRNA: 3'- -GC-GCUGAGGCaGGU--UGGGGGGUcauu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 162323 | 0.67 | 0.860965 |
Target: 5'- uCGCGugUCCGgUCGGCCCgUCCgaGGUAAg -3' miRNA: 3'- -GCGCugAGGCaGGUUGGG-GGG--UCAUU- -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 157071 | 0.67 | 0.845973 |
Target: 5'- cCGCcACcCUGUCCcaGGCCCCCCgcGGUGAg -3' miRNA: 3'- -GCGcUGaGGCAGG--UUGGGGGG--UCAUU- -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 221847 | 0.67 | 0.841334 |
Target: 5'- gCGCGGCg-CGUCCucGACCCCCUccucguugucgcuggGGUAGg -3' miRNA: 3'- -GCGCUGagGCAGG--UUGGGGGG---------------UCAUU- -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 151075 | 0.67 | 0.838207 |
Target: 5'- gCGCGGguUUCUGgaccgCCGACCCCUCGGg-- -3' miRNA: 3'- -GCGCU--GAGGCa----GGUUGGGGGGUCauu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 206607 | 0.67 | 0.838207 |
Target: 5'- gGCGACacgggcgCCGUCUAcGCCgCCCUGGUGGg -3' miRNA: 3'- gCGCUGa------GGCAGGU-UGG-GGGGUCAUU- -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 117392 | 0.67 | 0.838207 |
Target: 5'- gGCGcCUCCGUCCuAUCCCgCCgucGGUGc -3' miRNA: 3'- gCGCuGAGGCAGGuUGGGG-GG---UCAUu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 127478 | 0.67 | 0.83742 |
Target: 5'- -cCGGC-CCGUCCcccuggaAGCCCCCCGGc-- -3' miRNA: 3'- gcGCUGaGGCAGG-------UUGGGGGGUCauu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 208105 | 0.67 | 0.830269 |
Target: 5'- gGUGGCgUCG-CCAACCCCCCAc--- -3' miRNA: 3'- gCGCUGaGGCaGGUUGGGGGGUcauu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 85415 | 0.67 | 0.830269 |
Target: 5'- aGCaGGcCUCCGgugcCCAGCCCCCCGc--- -3' miRNA: 3'- gCG-CU-GAGGCa---GGUUGGGGGGUcauu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 187064 | 0.68 | 0.822165 |
Target: 5'- aCGCGACUCCGUCagcuuCUCCUCGa--- -3' miRNA: 3'- -GCGCUGAGGCAGguu--GGGGGGUcauu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 148593 | 0.68 | 0.813903 |
Target: 5'- gCGCGGCgcCCGUCgGGCCCUCaCAGc-- -3' miRNA: 3'- -GCGCUGa-GGCAGgUUGGGGG-GUCauu -5' |
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14182 | 5' | -58.4 | NC_003521.1 | + | 112806 | 0.69 | 0.761412 |
Target: 5'- aCGCG-CUCUGggacggCCGcgACCCCCCGGa-- -3' miRNA: 3'- -GCGCuGAGGCa-----GGU--UGGGGGGUCauu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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