Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14187 | 5' | -50.8 | NC_003521.1 | + | 140041 | 0.66 | 0.998455 |
Target: 5'- -cCUCUUcuugUUCgUCCUCCUcgCGCUACGa -3' miRNA: 3'- caGAGAG----AAG-AGGAGGAaaGUGGUGU- -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 22110 | 0.66 | 0.998278 |
Target: 5'- uUCUCgcccgccgccgccgCcgUCUCCUCCgccUUCGCCGCc -3' miRNA: 3'- cAGAGa-------------Ga-AGAGGAGGa--AAGUGGUGu -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 174603 | 0.66 | 0.99815 |
Target: 5'- aUCUUUCUauucagCUCCUCCUUcuuaGCCugGg -3' miRNA: 3'- cAGAGAGAa-----GAGGAGGAAag--UGGugU- -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 165291 | 0.66 | 0.997797 |
Target: 5'- cGUCUCUCUggcuguucuuccUCUUCUCCUgUCuCCGu- -3' miRNA: 3'- -CAGAGAGA------------AGAGGAGGAaAGuGGUgu -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 98639 | 0.66 | 0.997758 |
Target: 5'- cUC-CUCUUCuUCCUCCUcguccucUUCuuCCGCAg -3' miRNA: 3'- cAGaGAGAAG-AGGAGGA-------AAGu-GGUGU- -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 118380 | 0.66 | 0.997388 |
Target: 5'- gGUCUCUCUUCUUUUUCc--CACCGg- -3' miRNA: 3'- -CAGAGAGAAGAGGAGGaaaGUGGUgu -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 123857 | 0.67 | 0.996918 |
Target: 5'- -cCUUcCUUCUCCUCgUUUC-CCGCc -3' miRNA: 3'- caGAGaGAAGAGGAGgAAAGuGGUGu -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 95551 | 0.67 | 0.995768 |
Target: 5'- aUgUC-CUUCUCCUCCUgugaCAgCACAa -3' miRNA: 3'- cAgAGaGAAGAGGAGGAaa--GUgGUGU- -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 98539 | 0.68 | 0.993406 |
Target: 5'- cGUC-CUCUUCuUCCUCCga--GCCAUc -3' miRNA: 3'- -CAGaGAGAAG-AGGAGGaaagUGGUGu -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 84653 | 0.68 | 0.993406 |
Target: 5'- uGUCgugggUCUCCUCCgccUCGCCACc -3' miRNA: 3'- -CAGagagaAGAGGAGGaa-AGUGGUGu -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 191436 | 0.68 | 0.992417 |
Target: 5'- uGUCUCUCUcccUCUCCUUUgcgUUUCucguCCGCu -3' miRNA: 3'- -CAGAGAGA---AGAGGAGG---AAAGu---GGUGu -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 146328 | 0.68 | 0.991314 |
Target: 5'- cGUgUCUUUUCUCCccgaUCCggaggcaUCACCGCGc -3' miRNA: 3'- -CAgAGAGAAGAGG----AGGaa-----AGUGGUGU- -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 83733 | 0.68 | 0.990088 |
Target: 5'- ---cCUCgugCUCCUCC--UCACCGCGg -3' miRNA: 3'- cagaGAGaa-GAGGAGGaaAGUGGUGU- -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 67797 | 0.69 | 0.987234 |
Target: 5'- -gCUCUuCUUcCUCCUCCUcUCGCgGCu -3' miRNA: 3'- caGAGA-GAA-GAGGAGGAaAGUGgUGu -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 73624 | 0.69 | 0.981814 |
Target: 5'- aGUCUCUCUUgUUC-CCUUUCuauCUGCAg -3' miRNA: 3'- -CAGAGAGAAgAGGaGGAAAGu--GGUGU- -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 59941 | 0.7 | 0.974826 |
Target: 5'- cUCUCgcuggaCUUCUCCgUCUaUCGCCACAa -3' miRNA: 3'- cAGAGa-----GAAGAGGaGGAaAGUGGUGU- -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 129511 | 0.7 | 0.974826 |
Target: 5'- cGUCUCUCcUCUCUuacagUCCgg-CGCUACAg -3' miRNA: 3'- -CAGAGAGaAGAGG-----AGGaaaGUGGUGU- -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 42585 | 0.71 | 0.962711 |
Target: 5'- aGUCUcCUUUUCUCCUagUCUaUUACCACGg -3' miRNA: 3'- -CAGA-GAGAAGAGGA--GGAaAGUGGUGU- -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 85359 | 0.71 | 0.947035 |
Target: 5'- cGUCUCUCUcacccccuccuUCUCCUCCg--CcUCACAa -3' miRNA: 3'- -CAGAGAGA-----------AGAGGAGGaaaGuGGUGU- -5' |
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14187 | 5' | -50.8 | NC_003521.1 | + | 101587 | 0.72 | 0.937772 |
Target: 5'- cGUCUguccgaccCUCUUCUCCUCCUacucagugCACCAg- -3' miRNA: 3'- -CAGA--------GAGAAGAGGAGGAaa------GUGGUgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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