miRNA: 3'- -CCACGU-CUACCUCCACGug--GGa---GGGa -5'
miRNA: 3'- -CCACGUCUACcUCCa-CGUGGGa----GGGA- -5'
miRNA: 3'- -CCACGU-CUa--CCUCCACGUGGgaGGGa -5'
miRNA: 3'- -CCACGUCUACCUCCACGUGGGAGGGA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14209 | 5' | -58.5 | NC_003521.1 | + | 149021 | 0.75 | 0.420729 |
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Target: 5'- gGGUGCAgGGUGGGGGUGCccaaCCgccgCCCg -3' miRNA: 3'- -CCACGU-CUACCUCCACGug--GGa---GGGa -5' |
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| 14209 | 5' | -58.5 | NC_003521.1 | + | 141298 | 0.76 | 0.372025 |
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Target: 5'- aGGUGCAGGUGcGGGcgGCGCCCcggagCCCUc -3' miRNA: 3'- -CCACGUCUACcUCCa-CGUGGGa----GGGA- -5' |
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| 14209 | 5' | -58.5 | NC_003521.1 | + | 184165 | 0.8 | 0.212478 |
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Target: 5'- uGGUGCGcGAgcgGGAGGUGCACCggCCCg -3' miRNA: 3'- -CCACGU-CUa--CCUCCACGUGGgaGGGa -5' |
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| 14209 | 5' | -58.5 | NC_003521.1 | + | 67889 | 1.09 | 0.002703 |
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Target: 5'- aGGUGCAGAUGGAGGUGCACCCUCCCUc -3' miRNA: 3'- -CCACGUCUACCUCCACGUGGGAGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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