Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14214 | 5' | -57.3 | NC_003521.1 | + | 149403 | 0.66 | 0.907882 |
Target: 5'- cAGGCGcaugcgcacggucGCCCUGUGGGCcgggauuugGGUCg--- -3' miRNA: 3'- aUCCGCa------------CGGGAUACCCG---------CCAGauau -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 30562 | 0.66 | 0.904233 |
Target: 5'- aGGGUGUaGCCCUcgGGGUagggcaGGUCc--- -3' miRNA: 3'- aUCCGCA-CGGGAuaCCCG------CCAGauau -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 91568 | 0.66 | 0.891483 |
Target: 5'- cAGGUccGCCCacaagGGGCGGUgUGUGg -3' miRNA: 3'- aUCCGcaCGGGaua--CCCGCCAgAUAU- -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 154253 | 0.66 | 0.884775 |
Target: 5'- -cGGCGUGCCCUGccuccaguucUGGcagaaGGUCUGc- -3' miRNA: 3'- auCCGCACGGGAU----------ACCcg---CCAGAUau -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 126401 | 0.66 | 0.884093 |
Target: 5'- aGGGCGaaGCCCggguggaUGUGGGCcuuGGUCUGc- -3' miRNA: 3'- aUCCGCa-CGGG-------AUACCCG---CCAGAUau -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 12372 | 0.67 | 0.877851 |
Target: 5'- cUGGGUgccguuGUGCCaaCUAUgGGGCGGUCaUAUGa -3' miRNA: 3'- -AUCCG------CACGG--GAUA-CCCGCCAG-AUAU- -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 178805 | 0.67 | 0.877851 |
Target: 5'- gAGGCaUGCCCggcGGGCgGGUCg--- -3' miRNA: 3'- aUCCGcACGGGauaCCCG-CCAGauau -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 218379 | 0.67 | 0.870715 |
Target: 5'- cAGGCGUGCCgCgugaagcgcGGGCGGUaCUcgGc -3' miRNA: 3'- aUCCGCACGG-Gaua------CCCGCCA-GAuaU- -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 73000 | 0.67 | 0.863371 |
Target: 5'- -cGGuCGUGCCCg--GGGgGGUCc--- -3' miRNA: 3'- auCC-GCACGGGauaCCCgCCAGauau -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 176327 | 0.67 | 0.853524 |
Target: 5'- gAGGCGUccguagcgcuuuagGCCCUgaucgGUGGGCGGgUUGUc -3' miRNA: 3'- aUCCGCA--------------CGGGA-----UACCCGCCaGAUAu -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 176923 | 0.67 | 0.840153 |
Target: 5'- aUGGGCGgggGUCgUG-GGGCGGUCa--- -3' miRNA: 3'- -AUCCGCa--CGGgAUaCCCGCCAGauau -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 77730 | 0.69 | 0.761437 |
Target: 5'- -cGGCG-GCCUcgGUGGGCGGcgUCUGg- -3' miRNA: 3'- auCCGCaCGGGa-UACCCGCC--AGAUau -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 101799 | 0.69 | 0.742507 |
Target: 5'- uUGGGCGcgcacgaaGCCCaggGGGCGGUCgcgGUAg -3' miRNA: 3'- -AUCCGCa-------CGGGauaCCCGCCAGa--UAU- -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 22590 | 0.73 | 0.553578 |
Target: 5'- -cGGCcUGUCCUAccUGGGCGGUCUc-- -3' miRNA: 3'- auCCGcACGGGAU--ACCCGCCAGAuau -5' |
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14214 | 5' | -57.3 | NC_003521.1 | + | 64316 | 1.05 | 0.005221 |
Target: 5'- gUAGGCGUGCCCUAUGGGCGGUCUAUAu -3' miRNA: 3'- -AUCCGCACGGGAUACCCGCCAGAUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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