miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1424 3' -62.5 NC_001335.1 + 1839 0.66 0.327228
Target:  5'- --uGGGGCUGUGgGCCagccugucgaagaUCgAGGG-GCu -3'
miRNA:   3'- uagUCCCGACACgCGG-------------AGgUCCCgCG- -5'
1424 3' -62.5 NC_001335.1 + 7576 0.66 0.320299
Target:  5'- -cCGGGGUgucGgccgGCGCUUCCAGcGCGUc -3'
miRNA:   3'- uaGUCCCGa--Ca---CGCGGAGGUCcCGCG- -5'
1424 3' -62.5 NC_001335.1 + 4484 0.66 0.312729
Target:  5'- aGUCAGGGgUGUGUGCauacacgCCAGGaGuCGa -3'
miRNA:   3'- -UAGUCCCgACACGCGga-----GGUCC-C-GCg -5'
1424 3' -62.5 NC_001335.1 + 22142 0.67 0.286605
Target:  5'- aGUCcGGGaucaUGUcuuugaagcccugcaGCGCCUCCAGaGGCaGCu -3'
miRNA:   3'- -UAGuCCCg---ACA---------------CGCGGAGGUC-CCG-CG- -5'
1424 3' -62.5 NC_001335.1 + 5942 0.67 0.277603
Target:  5'- gGUC-GGGCUGgccUGUGCCguggacggugaacagCCAGGGCuuGCu -3'
miRNA:   3'- -UAGuCCCGAC---ACGCGGa--------------GGUCCCG--CG- -5'
1424 3' -62.5 NC_001335.1 + 18557 0.67 0.26354
Target:  5'- cUCcuGGCUGUcGCGagaCUCCgAGuGGCGCa -3'
miRNA:   3'- uAGucCCGACA-CGCg--GAGG-UC-CCGCG- -5'
1424 3' -62.5 NC_001335.1 + 32567 0.68 0.244461
Target:  5'- -cCAGGcGCUuccugaGCuGCCUgCGGGGCGCg -3'
miRNA:   3'- uaGUCC-CGAca----CG-CGGAgGUCCCGCG- -5'
1424 3' -62.5 NC_001335.1 + 132 0.69 0.19916
Target:  5'- cAUCccGGaauUGUcucaGCGCCUCuCAGGGCGCu -3'
miRNA:   3'- -UAGucCCg--ACA----CGCGGAG-GUCCCGCG- -5'
1424 3' -62.5 NC_001335.1 + 10279 0.71 0.133703
Target:  5'- -gCAGGGCUGggcgaggGuCGCCaCC-GGGCGCg -3'
miRNA:   3'- uaGUCCCGACa------C-GCGGaGGuCCCGCG- -5'
1424 3' -62.5 NC_001335.1 + 78 1.09 0.000145
Target:  5'- cAUCAGGGCUGUGCGCCUCCAGGGCGCg -3'
miRNA:   3'- -UAGUCCCGACACGCGGAGGUCCCGCG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.