Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14242 | 3' | -47.5 | NC_003521.1 | + | 195408 | 0.66 | 0.999985 |
Target: 5'- gCCGAcGGCGGGcaccggcUACCUGGUGa -3' miRNA: 3'- aGGCUuCCGUCUaaaau--AUGGGUCAC- -5' |
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14242 | 3' | -47.5 | NC_003521.1 | + | 229065 | 0.66 | 0.999985 |
Target: 5'- -aCGAAGGCAGuc-----ACCCAGg- -3' miRNA: 3'- agGCUUCCGUCuaaaauaUGGGUCac -5' |
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14242 | 3' | -47.5 | NC_003521.1 | + | 203214 | 0.66 | 0.999973 |
Target: 5'- aUCCGuGGGCAGcUUgaagucGCCCAGa- -3' miRNA: 3'- -AGGCuUCCGUCuAAaaua--UGGGUCac -5' |
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14242 | 3' | -47.5 | NC_003521.1 | + | 79160 | 0.66 | 0.999963 |
Target: 5'- cCCGAAGacguugacGCAGAUgcuguagccGUGCUCGGUGg -3' miRNA: 3'- aGGCUUC--------CGUCUAaaa------UAUGGGUCAC- -5' |
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14242 | 3' | -47.5 | NC_003521.1 | + | 3959 | 0.66 | 0.999963 |
Target: 5'- aUCCGAAGaaGCGGAgg----ACCCGGg- -3' miRNA: 3'- -AGGCUUC--CGUCUaaaauaUGGGUCac -5' |
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14242 | 3' | -47.5 | NC_003521.1 | + | 130978 | 0.66 | 0.999948 |
Target: 5'- -gUGggGGCAGugagcacgGUACCCAGc- -3' miRNA: 3'- agGCuuCCGUCuaaaa---UAUGGGUCac -5' |
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14242 | 3' | -47.5 | NC_003521.1 | + | 236253 | 0.69 | 0.999256 |
Target: 5'- aCCGGAGGCcGGcg-UGUGCCCcggccguAGUGa -3' miRNA: 3'- aGGCUUCCGuCUaaaAUAUGGG-------UCAC- -5' |
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14242 | 3' | -47.5 | NC_003521.1 | + | 119249 | 0.69 | 0.999105 |
Target: 5'- cUUGAacAGGUGGAUg--GUGCCCAGUa -3' miRNA: 3'- aGGCU--UCCGUCUAaaaUAUGGGUCAc -5' |
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14242 | 3' | -47.5 | NC_003521.1 | + | 77691 | 0.7 | 0.996772 |
Target: 5'- -aCGAGGGCAGAcag-AUGCCCgaggaggcggcGGUGg -3' miRNA: 3'- agGCUUCCGUCUaaaaUAUGGG-----------UCAC- -5' |
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14242 | 3' | -47.5 | NC_003521.1 | + | 157607 | 0.71 | 0.995549 |
Target: 5'- gCCGAGGGCAccg--UGUACCCcagcgAGUGg -3' miRNA: 3'- aGGCUUCCGUcuaaaAUAUGGG-----UCAC- -5' |
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14242 | 3' | -47.5 | NC_003521.1 | + | 120250 | 0.73 | 0.980615 |
Target: 5'- gCCGAAGGUGGAUccgUUAUcCCCGGg- -3' miRNA: 3'- aGGCUUCCGUCUAa--AAUAuGGGUCac -5' |
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14242 | 3' | -47.5 | NC_003521.1 | + | 240417 | 1.1 | 0.026552 |
Target: 5'- cUCCGAAGGCAGAUUUUAUACCCAGUGu -3' miRNA: 3'- -AGGCUUCCGUCUAAAAUAUGGGUCAC- -5' |
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14242 | 3' | -47.5 | NC_003521.1 | + | 40189 | 1.1 | 0.026552 |
Target: 5'- cUCCGAAGGCAGAUUUUAUACCCAGUGu -3' miRNA: 3'- -AGGCUUCCGUCUAAAAUAUGGGUCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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