Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14243 | 5' | -49.7 | NC_003521.1 | + | 105108 | 0.65 | 0.999542 |
Target: 5'- gGcGAGCGCGaGGUUUUgAgccuccuGAGGCAGAc -3' miRNA: 3'- gC-CUCGUGCaUCAAAAgU-------CUCCGUUU- -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 75555 | 0.66 | 0.999443 |
Target: 5'- aCGaGAGCGagacCGUGGUgugcgugUUCuGAGGCGAc -3' miRNA: 3'- -GC-CUCGU----GCAUCAa------AAGuCUCCGUUu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 78202 | 0.66 | 0.999443 |
Target: 5'- gCGGAGCgggcGCGgcGcucgUCGGAGGCu-- -3' miRNA: 3'- -GCCUCG----UGCauCaaa-AGUCUCCGuuu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 166909 | 0.66 | 0.999312 |
Target: 5'- aCGG-GCACG-GGg---CAGAGGUAGAc -3' miRNA: 3'- -GCCuCGUGCaUCaaaaGUCUCCGUUU- -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 46388 | 0.66 | 0.999312 |
Target: 5'- aGGGGCGCGgGGUg--CGuGGGGCGc- -3' miRNA: 3'- gCCUCGUGCaUCAaaaGU-CUCCGUuu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 186922 | 0.66 | 0.999312 |
Target: 5'- gGGGGCGCc-GGUgg-CGGGGGCGGc -3' miRNA: 3'- gCCUCGUGcaUCAaaaGUCUCCGUUu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 53773 | 0.66 | 0.998967 |
Target: 5'- aCGGAGuCGCGUcuccg-CGGAGGCGu- -3' miRNA: 3'- -GCCUC-GUGCAucaaaaGUCUCCGUuu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 74997 | 0.66 | 0.998967 |
Target: 5'- gCGGGuGCGCGUAGUgaUCGuGGGCc-- -3' miRNA: 3'- -GCCU-CGUGCAUCAaaAGUcUCCGuuu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 86986 | 0.66 | 0.998967 |
Target: 5'- uGGAGCGCGUccauccgUCAGAGcGUGGAa -3' miRNA: 3'- gCCUCGUGCAucaaa--AGUCUC-CGUUU- -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 170185 | 0.67 | 0.998746 |
Target: 5'- uCGGAGCG-GUGGUgccggUGGAGGUAGu -3' miRNA: 3'- -GCCUCGUgCAUCAaaa--GUCUCCGUUu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 113583 | 0.67 | 0.998486 |
Target: 5'- uGGGGCugGgcc---UCGGGGGCAAc -3' miRNA: 3'- gCCUCGugCaucaaaAGUCUCCGUUu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 6847 | 0.67 | 0.997827 |
Target: 5'- aGGAGguUGUAGUg---GGAGGCGu- -3' miRNA: 3'- gCCUCguGCAUCAaaagUCUCCGUuu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 155238 | 0.68 | 0.9964 |
Target: 5'- uGGAGUcCGUGGUgguggUgGGGGGCGGc -3' miRNA: 3'- gCCUCGuGCAUCAaa---AgUCUCCGUUu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 209588 | 0.68 | 0.995781 |
Target: 5'- gGGGcGCACGUAG----CGGAGGUAAAc -3' miRNA: 3'- gCCU-CGUGCAUCaaaaGUCUCCGUUU- -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 118684 | 0.68 | 0.995781 |
Target: 5'- uCGGGGCACGUGcugaaagccGUgUUCAGccgcGGCGAGa -3' miRNA: 3'- -GCCUCGUGCAU---------CAaAAGUCu---CCGUUU- -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 494 | 0.68 | 0.99428 |
Target: 5'- cCGaGAGaaCACGcGGUUUUCAGGGGUGAc -3' miRNA: 3'- -GC-CUC--GUGCaUCAAAAGUCUCCGUUu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 200721 | 0.68 | 0.99428 |
Target: 5'- cCGaGAGaaCACGcGGUUUUCAGGGGUGAc -3' miRNA: 3'- -GC-CUC--GUGCaUCAAAAGUCUCCGUUu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 180398 | 0.69 | 0.993382 |
Target: 5'- uGGGGC-UGUAGacg-UAGAGGCAGAg -3' miRNA: 3'- gCCUCGuGCAUCaaaaGUCUCCGUUU- -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 101344 | 0.69 | 0.992375 |
Target: 5'- gCGGuGUACGUAGUU----GAGGCAGc -3' miRNA: 3'- -GCCuCGUGCAUCAAaaguCUCCGUUu -5' |
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14243 | 5' | -49.7 | NC_003521.1 | + | 237737 | 0.69 | 0.990388 |
Target: 5'- gGGGGCcaugugagguaccACGUAGUUcccgggaucggagaaUUCGGGGGCGGu -3' miRNA: 3'- gCCUCG-------------UGCAUCAA---------------AAGUCUCCGUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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