Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1425 | 3' | -55.5 | NC_001335.1 | + | 1629 | 0.66 | 0.674652 |
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Target: 5'- cCCUC-CUcgAGGaCGCCugguucgGCGCAGGCa -3' miRNA: 3'- -GGAGaGAuaUCUcGCGG-------CGUGUCCGc -5' |
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| 1425 | 3' | -55.5 | NC_001335.1 | + | 52217 | 0.66 | 0.664782 |
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Target: 5'- aCUCgaugCgaugcgAGAGCGCCGCgGCAcGCGg -3' miRNA: 3'- gGAGa---Gaua---UCUCGCGGCG-UGUcCGC- -5' |
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| 1425 | 3' | -55.5 | NC_001335.1 | + | 19526 | 0.67 | 0.642771 |
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Target: 5'- aCCcgCUCgcc--AGCgaccagGCCGCGCAGGCGg -3' miRNA: 3'- -GGa-GAGauaucUCG------CGGCGUGUCCGC- -5' |
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| 1425 | 3' | -55.5 | NC_001335.1 | + | 28666 | 0.67 | 0.603107 |
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Target: 5'- ------gAUGGAGCGCCGagugcgccaccuuccCGCAGGCGa -3' miRNA: 3'- ggagagaUAUCUCGCGGC---------------GUGUCCGC- -5' |
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| 1425 | 3' | -55.5 | NC_001335.1 | + | 3777 | 0.68 | 0.587745 |
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Target: 5'- aCCUCaUCgaacGAGCGCCGCG-AGGUc -3' miRNA: 3'- -GGAG-AGauauCUCGCGGCGUgUCCGc -5' |
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| 1425 | 3' | -55.5 | NC_001335.1 | + | 46210 | 0.68 | 0.543261 |
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Target: 5'- cCCgCUCUGUgaguaccGGAGCGCagCGCACAucgccgagcacGGCGg -3' miRNA: 3'- -GGaGAGAUA-------UCUCGCG--GCGUGU-----------CCGC- -5' |
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| 1425 | 3' | -55.5 | NC_001335.1 | + | 452 | 0.75 | 0.24066 |
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Target: 5'- aCCcCUCUAUAGGGC-CgCGCACuGGCGc -3' miRNA: 3'- -GGaGAGAUAUCUCGcG-GCGUGuCCGC- -5' |
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| 1425 | 3' | -55.5 | NC_001335.1 | + | 249 | 1.12 | 0.00059 |
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Target: 5'- cCCUCUCUAUAGAGCGCCGCACAGGCGg -3' miRNA: 3'- -GGAGAGAUAUCUCGCGGCGUGUCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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