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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14252 | 3' | -56.4 | NC_003521.1 | + | 92080 | 0.7 | 0.820694 |
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Target: 5'- aCCGGGgGUCggGGGgUGCGCAG-GGc -3' miRNA: 3'- -GGCCUgUAGagCCCgAUGCGUCaCCu -5' |
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| 14252 | 3' | -56.4 | NC_003521.1 | + | 78656 | 0.67 | 0.928158 |
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Target: 5'- --cGAUGUCgUCGGGCUucucgGCGCAGgGGAa -3' miRNA: 3'- ggcCUGUAG-AGCCCGA-----UGCGUCaCCU- -5' |
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| 14252 | 3' | -56.4 | NC_003521.1 | + | 60127 | 0.67 | 0.933093 |
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Target: 5'- aUGGGCAUCUugUGGGUUcGCGUuuugguaaccuGGUGGAu -3' miRNA: 3'- gGCCUGUAGA--GCCCGA-UGCG-----------UCACCU- -5' |
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| 14252 | 3' | -56.4 | NC_003521.1 | + | 39566 | 0.67 | 0.946587 |
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Target: 5'- gCCGGcCAUCU--GGCUGCGCGGcGuGAu -3' miRNA: 3'- -GGCCuGUAGAgcCCGAUGCGUCaC-CU- -5' |
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| 14252 | 3' | -56.4 | NC_003521.1 | + | 32115 | 0.66 | 0.964831 |
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Target: 5'- gCGcGGCugAUCUCGGGCagguagcggGCGCGGUGc- -3' miRNA: 3'- gGC-CUG--UAGAGCCCGa--------UGCGUCACcu -5' |
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| 14252 | 3' | -56.4 | NC_003521.1 | + | 29067 | 1.11 | 0.003821 |
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Target: 5'- gCCGGACAUCUCGGGCUACGCAGUGGAg -3' miRNA: 3'- -GGCCUGUAGAGCCCGAUGCGUCACCU- -5' |
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| 14252 | 3' | -56.4 | NC_003521.1 | + | 21935 | 0.81 | 0.293174 |
|
Target: 5'- gCGGGCGgcCUCGGGCUGgaGCAGUGGGc -3' miRNA: 3'- gGCCUGUa-GAGCCCGAUg-CGUCACCU- -5' |
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| 14252 | 3' | -56.4 | NC_003521.1 | + | 7756 | 0.66 | 0.954507 |
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Target: 5'- gCCGGACugcugAUCaUUGuGGCUuCGCuAGUGGGa -3' miRNA: 3'- -GGCCUG-----UAG-AGC-CCGAuGCG-UCACCU- -5' |
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| 14252 | 3' | -56.4 | NC_003521.1 | + | 5547 | 0.69 | 0.876758 |
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Target: 5'- gCGGcGCGUCguuccucccgguugCGGGCUcgGCGGUGGAa -3' miRNA: 3'- gGCC-UGUAGa-------------GCCCGAugCGUCACCU- -5' |
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| 14252 | 3' | -56.4 | NC_003521.1 | + | 97 | 0.66 | 0.953753 |
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Target: 5'- gUCGGACGUguUUCGGGCcggcgggucguCGCGG-GGAc -3' miRNA: 3'- -GGCCUGUA--GAGCCCGau---------GCGUCaCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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