Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1426 | 3' | -48 | NC_001335.1 | + | 32384 | 0.66 | 0.975193 |
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Target: 5'- --cCGGC-UUCUCGACCUcgGUGGAUGu -3' miRNA: 3'- caaGCUGcAAGAGUUGGA--CGCUUACu -5' |
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| 1426 | 3' | -48 | NC_001335.1 | + | 10228 | 0.68 | 0.943365 |
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Target: 5'- -cUCGGCGgagagUCGGCCUGCGAc--- -3' miRNA: 3'- caAGCUGCaag--AGUUGGACGCUuacu -5' |
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| 1426 | 3' | -48 | NC_001335.1 | + | 40035 | 0.68 | 0.943365 |
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Target: 5'- -gUCGACGUUCUCcucggaGACgUUGCGGGcGAg -3' miRNA: 3'- caAGCUGCAAGAG------UUG-GACGCUUaCU- -5' |
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| 1426 | 3' | -48 | NC_001335.1 | + | 1581 | 0.69 | 0.913849 |
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Target: 5'- uGUUCGAgGUcggcuUCUCAGCCggugGCGAc--- -3' miRNA: 3'- -CAAGCUgCA-----AGAGUUGGa---CGCUuacu -5' |
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| 1426 | 3' | -48 | NC_001335.1 | + | 14291 | 0.7 | 0.884749 |
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Target: 5'- ---gGugGUUCUCggUCUGCGGAc-- -3' miRNA: 3'- caagCugCAAGAGuuGGACGCUUacu -5' |
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| 1426 | 3' | -48 | NC_001335.1 | + | 29967 | 0.75 | 0.625016 |
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Target: 5'- -cUUGACGUUCUCGGCCUGguccuCGGAgaUGAa -3' miRNA: 3'- caAGCUGCAAGAGUUGGAC-----GCUU--ACU- -5' |
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| 1426 | 3' | -48 | NC_001335.1 | + | 457 | 1.1 | 0.004851 |
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Target: 5'- aGUUCGACGUUCUCAACCUGCGAAUGAc -3' miRNA: 3'- -CAAGCUGCAAGAGUUGGACGCUUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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