Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1427 | 5' | -51.7 | NC_001335.1 | + | 34731 | 0.66 | 0.84272 |
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Target: 5'- aUGGAGGgcggGGGAGuCGgGGCGAAcUACu- -3' miRNA: 3'- gACCUCU----UCUUC-GCgCCGCUU-AUGuu -5' |
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| 1427 | 5' | -51.7 | NC_001335.1 | + | 28670 | 0.67 | 0.804492 |
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Target: 5'- -aGGAGAuGGAGCGC--CGAGUGCGc -3' miRNA: 3'- gaCCUCUuCUUCGCGccGCUUAUGUu -5' |
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| 1427 | 5' | -51.7 | NC_001335.1 | + | 43166 | 0.67 | 0.772657 |
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Target: 5'- -cGGGGAAGAAGgGCaucaaGGCGAAgcccacugcgcccUACGAc -3' miRNA: 3'- gaCCUCUUCUUCgCG-----CCGCUU-------------AUGUU- -5' |
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| 1427 | 5' | -51.7 | NC_001335.1 | + | 26438 | 0.68 | 0.763113 |
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Target: 5'- -cGGcaucGGAGGAGGCGCGacCGggUACGg -3' miRNA: 3'- gaCC----UCUUCUUCGCGCc-GCuuAUGUu -5' |
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| 1427 | 5' | -51.7 | NC_001335.1 | + | 43509 | 0.68 | 0.762045 |
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Target: 5'- aCUGGAGcagguaGAGAAguucaucGCGUGGCGGAaggACAAg -3' miRNA: 3'- -GACCUC------UUCUU-------CGCGCCGCUUa--UGUU- -5' |
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| 1427 | 5' | -51.7 | NC_001335.1 | + | 39316 | 0.69 | 0.708184 |
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Target: 5'- -gGGAGGAGGAcGCGCGuGcCGAGggcgGCGAa -3' miRNA: 3'- gaCCUCUUCUU-CGCGC-C-GCUUa---UGUU- -5' |
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| 1427 | 5' | -51.7 | NC_001335.1 | + | 39921 | 0.69 | 0.68555 |
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Target: 5'- -gGGAGgcGAucuGaUGCGGCGAGUACu- -3' miRNA: 3'- gaCCUCuuCUu--C-GCGCCGCUUAUGuu -5' |
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| 1427 | 5' | -51.7 | NC_001335.1 | + | 1318 | 1.06 | 0.002845 |
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Target: 5'- gCUGGAGAAGAAGCGCGGCGAAUACAAc -3' miRNA: 3'- -GACCUCUUCUUCGCGCCGCUUAUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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