Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1429 | 3' | -55.1 | NC_001335.1 | + | 43594 | 0.67 | 0.645162 |
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Target: 5'- -cGUUCGAGGcucaucucgCGGCc-CUCGGCCa- -3' miRNA: 3'- gaCAAGCUCCa--------GCCGaaGAGUCGGcc -5' |
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| 1429 | 3' | -55.1 | NC_001335.1 | + | 27295 | 0.68 | 0.634193 |
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Target: 5'- ----gCGAGGUUGGC--CUCGGCgCGGu -3' miRNA: 3'- gacaaGCUCCAGCCGaaGAGUCG-GCC- -5' |
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| 1429 | 3' | -55.1 | NC_001335.1 | + | 11905 | 0.69 | 0.547143 |
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Target: 5'- ----gUGAGGUCGGCgUUggAGCCGGu -3' miRNA: 3'- gacaaGCUCCAGCCGaAGagUCGGCC- -5' |
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| 1429 | 3' | -55.1 | NC_001335.1 | + | 2314 | 0.7 | 0.515373 |
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Target: 5'- ----gCGAGGUCGGCaaggagugagCUgGGCCGGc -3' miRNA: 3'- gacaaGCUCCAGCCGaa--------GAgUCGGCC- -5' |
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| 1429 | 3' | -55.1 | NC_001335.1 | + | 1694 | 0.7 | 0.484376 |
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Target: 5'- -cGUccUCGAGGagGGCgcgUUCGGUCGGg -3' miRNA: 3'- gaCA--AGCUCCagCCGaa-GAGUCGGCC- -5' |
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| 1429 | 3' | -55.1 | NC_001335.1 | + | 52058 | 0.71 | 0.451348 |
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Target: 5'- gCUGUgugUCGAGGcCGGCUcucgcaucgucgcaUgUCAGCgCGGg -3' miRNA: 3'- -GACA---AGCUCCaGCCGA--------------AgAGUCG-GCC- -5' |
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| 1429 | 3' | -55.1 | NC_001335.1 | + | 1579 | 1.1 | 0.000936 |
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Target: 5'- gCUGUUCGAGGUCGGCUUCUCAGCCGGu -3' miRNA: 3'- -GACAAGCUCCAGCCGAAGAGUCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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