miRNA: 3'- -GGGGCuGGCUUG-CGC-GGG--AGGAGCUC- -5'
miRNA: 3'- -GGGGC-------------UGGC--UUGCGCGG----GAGGAGCUC- -5'
miRNA: 3'- -GGGGCUGGCUUGCGCGGGAGGAGCUC- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1429 | 5' | -60.5 | NC_001335.1 | + | 11245 | 0.72 | 0.212297 |
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Target: 5'- aUCCCG-CUGAACgGUGaCCCgaUCCUCGAGg -3' miRNA: 3'- -GGGGCuGGCUUG-CGC-GGG--AGGAGCUC- -5' |
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| 1429 | 5' | -60.5 | NC_001335.1 | + | 21153 | 0.72 | 0.196242 |
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Target: 5'- uCCCCGcagugccaagagcGCCGuuGACGCGCUugauCUCUUCGAGg -3' miRNA: 3'- -GGGGC-------------UGGC--UUGCGCGG----GAGGAGCUC- -5' |
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| 1429 | 5' | -60.5 | NC_001335.1 | + | 1613 | 1.11 | 0.000286 |
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Target: 5'- aCCCCGACCGAACGCGCCCUCCUCGAGg -3' miRNA: 3'- -GGGGCUGGCUUGCGCGGGAGGAGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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