Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14302 | 5' | -51.4 | NC_003521.1 | + | 49992 | 0.71 | 0.934474 |
Target: 5'- ----aGGGAGCCGGGUcguccagcgagGAcaagGAGGAAAGg -3' miRNA: 3'- cuguaCCCUCGGUCCA-----------CU----UUCCUUUC- -5' |
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14302 | 5' | -51.4 | NC_003521.1 | + | 98227 | 0.72 | 0.918282 |
Target: 5'- cGGCGUGGGAGgCGGG-GAGGcGGggGc -3' miRNA: 3'- -CUGUACCCUCgGUCCaCUUU-CCuuUc -5' |
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14302 | 5' | -51.4 | NC_003521.1 | + | 233555 | 0.72 | 0.906266 |
Target: 5'- cGACGUGGGAucCCAGGUGAcgauccGGGAGc- -3' miRNA: 3'- -CUGUACCCUc-GGUCCACUu-----UCCUUuc -5' |
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14302 | 5' | -51.4 | NC_003521.1 | + | 195351 | 0.78 | 0.658979 |
Target: 5'- cGugGUGGuGGGCCGGGUGAAGGuGAcgcccGAGg -3' miRNA: 3'- -CugUACC-CUCGGUCCACUUUC-CU-----UUC- -5' |
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14302 | 5' | -51.4 | NC_003521.1 | + | 4189 | 1.1 | 0.009975 |
Target: 5'- gGACAUGGGAGCCAGGUGAAAGGAAAGg -3' miRNA: 3'- -CUGUACCCUCGGUCCACUUUCCUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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