Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14308 | 5' | -54.8 | NC_003521.1 | + | 102964 | 0.7 | 0.897052 |
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Target: 5'- cUCGGGCgUCGGGGcucugGCGGGGcgGcccagccGCGGc -3' miRNA: 3'- aAGCUUG-AGCCCC-----UGCCCCuaU-------UGCC- -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 148380 | 0.7 | 0.903855 |
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Target: 5'- -cCGAGCggggugCGGGGAggcugGGGGGUGAgGGc -3' miRNA: 3'- aaGCUUGa-----GCCCCUg----CCCCUAUUgCC- -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 100368 | 0.69 | 0.909809 |
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Target: 5'- -gCGGACgggugaCGGGGAcggcacggaggcCGGGGAgaaGACGGg -3' miRNA: 3'- aaGCUUGa-----GCCCCU------------GCCCCUa--UUGCC- -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 43571 | 0.69 | 0.909809 |
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Target: 5'- -cCGcuGACUgGgGGGACGGGGGcaaAACGGa -3' miRNA: 3'- aaGC--UUGAgC-CCCUGCCCCUa--UUGCC- -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 90876 | 0.69 | 0.915542 |
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Target: 5'- -cCGAG---GGGGACGGGGAggggGACGa -3' miRNA: 3'- aaGCUUgagCCCCUGCCCCUa---UUGCc -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 182599 | 0.68 | 0.940855 |
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Target: 5'- -aCGggUuUCGGGaagaaGACGGGGAUaaucugggagGACGGg -3' miRNA: 3'- aaGCuuG-AGCCC-----CUGCCCCUA----------UUGCC- -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 94577 | 0.68 | 0.940855 |
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Target: 5'- -gUGAACUgCGGGucaaagaccACGGGGGUGACGa -3' miRNA: 3'- aaGCUUGA-GCCCc--------UGCCCCUAUUGCc -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 156691 | 0.73 | 0.754364 |
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Target: 5'- cUUCGcGCUCGGGGAaaaggugcuggcCGGGGAggAGCGc -3' miRNA: 3'- -AAGCuUGAGCCCCU------------GCCCCUa-UUGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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