Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14308 | 5' | -54.8 | NC_003521.1 | + | 113588 | 0.67 | 0.967104 |
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Target: 5'- -cUGGGcCUCGGGGgcaACGGGGGcGGCGc -3' miRNA: 3'- aaGCUU-GAGCCCC---UGCCCCUaUUGCc -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 148653 | 0.67 | 0.960654 |
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Target: 5'- -cCGAGCUCGG---CGGGGAUGGCc- -3' miRNA: 3'- aaGCUUGAGCCccuGCCCCUAUUGcc -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 120956 | 0.68 | 0.953379 |
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Target: 5'- --gGGGCa-GGGGGCGGGGcgGuuuGCGGu -3' miRNA: 3'- aagCUUGagCCCCUGCCCCuaU---UGCC- -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 178830 | 0.68 | 0.949015 |
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Target: 5'- -gUGAACUCGcucaggcGGcGCGGGGGcGACGGg -3' miRNA: 3'- aaGCUUGAGC-------CCcUGCCCCUaUUGCC- -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 169200 | 0.68 | 0.945248 |
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Target: 5'- -gUGGACgggCGGGGACGGaGGAgacAACa- -3' miRNA: 3'- aaGCUUGa--GCCCCUGCC-CCUa--UUGcc -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 99167 | 0.68 | 0.938112 |
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Target: 5'- gUCGAGCUCugcuucuccggccugGGGGcugaagguggcgGCGGaGGAUAcgGCGGg -3' miRNA: 3'- aAGCUUGAG---------------CCCC------------UGCC-CCUAU--UGCC- -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 228441 | 0.68 | 0.936239 |
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Target: 5'- -aCGAACUCGGcgugaaagaGGcCGGaGGGUAGCGa -3' miRNA: 3'- aaGCUUGAGCC---------CCuGCC-CCUAUUGCc -5' |
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| 14308 | 5' | -54.8 | NC_003521.1 | + | 10464 | 1.09 | 0.007328 |
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Target: 5'- uUUCGAACUCGGGGACGGGGAUAACGGa -3' miRNA: 3'- -AAGCUUGAGCCCCUGCCCCUAUUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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