Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14309 | 3' | -50.5 | NC_003521.1 | + | 126624 | 0.66 | 0.999305 |
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Target: 5'- uGUAGCGcUUGAUGUCgUCGUUgaUGCCc -3' miRNA: 3'- -CAUUGC-AACUGCAGgGGCAAg-AUGGu -5' |
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| 14309 | 3' | -50.5 | NC_003521.1 | + | 150774 | 0.66 | 0.999276 |
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Target: 5'- --cGCGUcGGCGUCCCgGagcuggaggccgUCUACCGc -3' miRNA: 3'- cauUGCAaCUGCAGGGgCa-----------AGAUGGU- -5' |
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| 14309 | 3' | -50.5 | NC_003521.1 | + | 47999 | 0.67 | 0.997867 |
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Target: 5'- cGUGACGUUGuuGgaaCCgCCGUUC-ACCGu -3' miRNA: 3'- -CAUUGCAACugCa--GG-GGCAAGaUGGU- -5' |
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| 14309 | 3' | -50.5 | NC_003521.1 | + | 7096 | 0.67 | 0.997474 |
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Target: 5'- -cGGCGgcGGCGUCCUCcUUCU-CCAg -3' miRNA: 3'- caUUGCaaCUGCAGGGGcAAGAuGGU- -5' |
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| 14309 | 3' | -50.5 | NC_003521.1 | + | 113466 | 0.68 | 0.996505 |
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Target: 5'- -aGACGUUGACGUUCUCGcagcUCUuucCCGg -3' miRNA: 3'- caUUGCAACUGCAGGGGCa---AGAu--GGU- -5' |
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| 14309 | 3' | -50.5 | NC_003521.1 | + | 152082 | 0.69 | 0.989165 |
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Target: 5'- --uGCGUgaUGGCGUCCUCGgUCU-CCAg -3' miRNA: 3'- cauUGCA--ACUGCAGGGGCaAGAuGGU- -5' |
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| 14309 | 3' | -50.5 | NC_003521.1 | + | 167289 | 0.7 | 0.98615 |
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Target: 5'- -gAGCG-UGAUGUUCUCGUUCU-CCAc -3' miRNA: 3'- caUUGCaACUGCAGGGGCAAGAuGGU- -5' |
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| 14309 | 3' | -50.5 | NC_003521.1 | + | 170055 | 0.7 | 0.984419 |
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Target: 5'- aUGAgGUUGGCGUUCCCGgucgcgUUCU-CCAg -3' miRNA: 3'- cAUUgCAACUGCAGGGGC------AAGAuGGU- -5' |
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| 14309 | 3' | -50.5 | NC_003521.1 | + | 9965 | 0.74 | 0.901381 |
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Target: 5'- ------aUGACGUCCCCG-UCUGCCc -3' miRNA: 3'- cauugcaACUGCAGGGGCaAGAUGGu -5' |
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| 14309 | 3' | -50.5 | NC_003521.1 | + | 10649 | 1.09 | 0.016019 |
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Target: 5'- cGUAACGUUGACGUCCCCGUUCUACCAc -3' miRNA: 3'- -CAUUGCAACUGCAGGGGCAAGAUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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