Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1432 | 3' | -53.4 | NC_001335.1 | + | 51826 | 0.7 | 0.596087 |
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Target: 5'- cGCGGCAcGGGCUGCC--GGGACUGu -3' miRNA: 3'- cCGCUGUcCUUGAUGGucUCCUGACc -5' |
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| 1432 | 3' | -53.4 | NC_001335.1 | + | 29018 | 0.7 | 0.585073 |
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Target: 5'- cGCGAUGGGccggcGGCUGCCGGuGGACa-- -3' miRNA: 3'- cCGCUGUCC-----UUGAUGGUCuCCUGacc -5' |
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| 1432 | 3' | -53.4 | NC_001335.1 | + | 23135 | 0.73 | 0.428709 |
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Target: 5'- cGCGAUGGGugcgAACUACCGccgcuGGGGCUGGg -3' miRNA: 3'- cCGCUGUCC----UUGAUGGUc----UCCUGACC- -5' |
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| 1432 | 3' | -53.4 | NC_001335.1 | + | 32884 | 0.73 | 0.409699 |
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Target: 5'- aGGCu-CAGGAGCcgggGCCGGcucGGGCUGGg -3' miRNA: 3'- -CCGcuGUCCUUGa---UGGUCu--CCUGACC- -5' |
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| 1432 | 3' | -53.4 | NC_001335.1 | + | 1828 | 0.74 | 0.359367 |
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Target: 5'- uGGCGACGgcguGGGGCUguggGCCAGccugucgaagaucgaGGGGCUGGu -3' miRNA: 3'- -CCGCUGU----CCUUGA----UGGUC---------------UCCUGACC- -5' |
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| 1432 | 3' | -53.4 | NC_001335.1 | + | 2351 | 1.14 | 0.000719 |
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Target: 5'- cGGCGACAGGAACUACCAGAGGACUGGg -3' miRNA: 3'- -CCGCUGUCCUUGAUGGUCUCCUGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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