Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1432 | 5' | -57.2 | NC_001335.1 | + | 21498 | 0.66 | 0.603888 |
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Target: 5'- cACgGGCUCUgGGUCaggaUCGUCuuUGCc -3' miRNA: 3'- aUGgCCGAGGgCCAGgaa-AGCAG--ACG- -5' |
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| 1432 | 5' | -57.2 | NC_001335.1 | + | 41481 | 0.66 | 0.582174 |
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Target: 5'- cGCCGGC-CCCGGcaCCgggaaGUCgGCg -3' miRNA: 3'- aUGGCCGaGGGCCa-GGaaag-CAGaCG- -5' |
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| 1432 | 5' | -57.2 | NC_001335.1 | + | 7359 | 0.67 | 0.539298 |
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Target: 5'- gACCaaggucgagGGCUCCCGcacCCUgUCGaUCUGCa -3' miRNA: 3'- aUGG---------CCGAGGGCca-GGAaAGC-AGACG- -5' |
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| 1432 | 5' | -57.2 | NC_001335.1 | + | 39830 | 0.67 | 0.528738 |
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Target: 5'- cACCGGUccuUCuCCGGUCUcacggUCGUCcacgGCg -3' miRNA: 3'- aUGGCCG---AG-GGCCAGGaa---AGCAGa---CG- -5' |
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| 1432 | 5' | -57.2 | NC_001335.1 | + | 13128 | 0.68 | 0.487331 |
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Target: 5'- cACCGGUgCCCGGUaccagaCCUgcaUCGUCUa- -3' miRNA: 3'- aUGGCCGaGGGCCA------GGAa--AGCAGAcg -5' |
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| 1432 | 5' | -57.2 | NC_001335.1 | + | 32957 | 0.68 | 0.467207 |
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Target: 5'- aGCCGGC-CCCGGcUCCUga-GcCUGa -3' miRNA: 3'- aUGGCCGaGGGCC-AGGAaagCaGACg -5' |
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| 1432 | 5' | -57.2 | NC_001335.1 | + | 16753 | 0.68 | 0.467207 |
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Target: 5'- aACCGGCUCauGGagUCCg---GUCUGCa -3' miRNA: 3'- aUGGCCGAGggCC--AGGaaagCAGACG- -5' |
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| 1432 | 5' | -57.2 | NC_001335.1 | + | 36322 | 0.68 | 0.453378 |
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Target: 5'- gGCCGGUUCUCGGUCgacggccgaGUCUGg -3' miRNA: 3'- aUGGCCGAGGGCCAGgaaag----CAGACg -5' |
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| 1432 | 5' | -57.2 | NC_001335.1 | + | 2386 | 0.93 | 0.008478 |
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Target: 5'- uUACCGGCUCCCGGUCCUUUCuG-CUGCc -3' miRNA: 3'- -AUGGCCGAGGGCCAGGAAAG-CaGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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