Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1435 | 3' | -57.4 | NC_001335.1 | + | 38900 | 0.72 | 0.286552 |
Target: 5'- aCGGGCcaucGAGUACCUGCUGaACCCGcCGg -3' miRNA: 3'- aGCUUG----CUCGUGGGCGAC-UGGGCuGU- -5' |
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1435 | 3' | -57.4 | NC_001335.1 | + | 50710 | 0.73 | 0.228163 |
Target: 5'- cUCGAggcaACGGGUACguccguauCCGCUGACuuGACAg -3' miRNA: 3'- -AGCU----UGCUCGUG--------GGCGACUGggCUGU- -5' |
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1435 | 3' | -57.4 | NC_001335.1 | + | 30015 | 0.74 | 0.216637 |
Target: 5'- uUCGAGCGAGUGCCCGa--ACCCGcCGg -3' miRNA: 3'- -AGCUUGCUCGUGGGCgacUGGGCuGU- -5' |
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1435 | 3' | -57.4 | NC_001335.1 | + | 29536 | 0.74 | 0.205612 |
Target: 5'- gUCGAGCaGAGC-CgCGUUGACCUGGCGc -3' miRNA: 3'- -AGCUUG-CUCGuGgGCGACUGGGCUGU- -5' |
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1435 | 3' | -57.4 | NC_001335.1 | + | 3034 | 1.09 | 0.000634 |
Target: 5'- gUCGAACGAGCACCCGCUGACCCGACAg -3' miRNA: 3'- -AGCUUGCUCGUGGGCGACUGGGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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