Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1436 | 5' | -58.8 | NC_001335.1 | + | 4901 | 0.66 | 0.540933 |
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Target: 5'- aGGcCGCuGGCCcgccucGGGGCaUCCGCUuGAa -3' miRNA: 3'- -CCaGUGuCCGG------CCCUGaAGGCGAcCU- -5' |
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| 1436 | 5' | -58.8 | NC_001335.1 | + | 17179 | 0.66 | 0.499785 |
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Target: 5'- uGGUCAcCAGuaCGucGGAgaUCUGCUGGAa -3' miRNA: 3'- -CCAGU-GUCcgGC--CCUgaAGGCGACCU- -5' |
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| 1436 | 5' | -58.8 | NC_001335.1 | + | 49423 | 0.68 | 0.431433 |
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Target: 5'- uGGUUACAGcGCgUGGGACUgccaaCGCUGcGGc -3' miRNA: 3'- -CCAGUGUC-CG-GCCCUGAag---GCGAC-CU- -5' |
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| 1436 | 5' | -58.8 | NC_001335.1 | + | 12730 | 0.68 | 0.407466 |
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Target: 5'- cGUC-CAGGUCGGGgauccggcGCUugaucaugcgcucgaUCUGCUGGAg -3' miRNA: 3'- cCAGuGUCCGGCCC--------UGA---------------AGGCGACCU- -5' |
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| 1436 | 5' | -58.8 | NC_001335.1 | + | 17545 | 0.68 | 0.394911 |
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Target: 5'- -uUCGCAGcgaucuGCUGGG-C-UCCGCUGGAg -3' miRNA: 3'- ccAGUGUC------CGGCCCuGaAGGCGACCU- -5' |
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| 1436 | 5' | -58.8 | NC_001335.1 | + | 29024 | 0.69 | 0.368865 |
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Target: 5'- cGUCAacgcgauGGGCCGGcGGCUgCCGgUGGAc -3' miRNA: 3'- cCAGUg------UCCGGCC-CUGAaGGCgACCU- -5' |
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| 1436 | 5' | -58.8 | NC_001335.1 | + | 16770 | 0.69 | 0.368865 |
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Target: 5'- cGGUCuGCAGGCgaUGGGACagcucggcggUCCGCUGu- -3' miRNA: 3'- -CCAG-UGUCCG--GCCCUGa---------AGGCGACcu -5' |
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| 1436 | 5' | -58.8 | NC_001335.1 | + | 19283 | 0.7 | 0.312807 |
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Target: 5'- cGGcUCACgAGGCCGuGGuCgcgCUGCUGGGg -3' miRNA: 3'- -CC-AGUG-UCCGGC-CCuGaa-GGCGACCU- -5' |
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| 1436 | 5' | -58.8 | NC_001335.1 | + | 3249 | 1.1 | 0.000392 |
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Target: 5'- uGGUCACAGGCCGGGACUUCCGCUGGAc -3' miRNA: 3'- -CCAGUGUCCGGCCCUGAAGGCGACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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