Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14360 | 3' | -56.5 | NC_003521.1 | + | 168677 | 0.66 | 0.94386 |
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Target: 5'- -cGGAGgUCCCUGGUGGcagGCcgUCCucGGUc -3' miRNA: 3'- gaCCUC-AGGGACCACC---UGa-AGGu-CUA- -5' |
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| 14360 | 3' | -56.5 | NC_003521.1 | + | 64077 | 0.67 | 0.887869 |
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Target: 5'- gCUGG-GUCugugcgagcccggCCUGGaggcGGGCUUCCAGAa -3' miRNA: 3'- -GACCuCAG-------------GGACCa---CCUGAAGGUCUa -5' |
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| 14360 | 3' | -56.5 | NC_003521.1 | + | 193748 | 0.68 | 0.881776 |
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Target: 5'- -aGGAGUUCCUGcgcGUGGGCUUUCGu-- -3' miRNA: 3'- gaCCUCAGGGAC---CACCUGAAGGUcua -5' |
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| 14360 | 3' | -56.5 | NC_003521.1 | + | 167120 | 0.7 | 0.794647 |
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Target: 5'- -aGGAGccUCCCUGG-GGACUUCgcuUAGAg -3' miRNA: 3'- gaCCUC--AGGGACCaCCUGAAG---GUCUa -5' |
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| 14360 | 3' | -56.5 | NC_003521.1 | + | 120094 | 0.73 | 0.630987 |
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Target: 5'- cCUGGuGcCCCUGGUGGccACggUCCAGGg -3' miRNA: 3'- -GACCuCaGGGACCACC--UGa-AGGUCUa -5' |
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| 14360 | 3' | -56.5 | NC_003521.1 | + | 59632 | 0.76 | 0.448205 |
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Target: 5'- cCUGGAGcCCCgGGUGGACUUCguGc- -3' miRNA: 3'- -GACCUCaGGGaCCACCUGAAGguCua -5' |
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| 14360 | 3' | -56.5 | NC_003521.1 | + | 44043 | 1.05 | 0.006844 |
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Target: 5'- gCUGGAGUCCCUGGUGGACUUCCAGAUc -3' miRNA: 3'- -GACCUCAGGGACCACCUGAAGGUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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