miRNA: 3'- caGGGUCUCCAG-GUGCU--UCU---UCGCGU- -5'
miRNA: 3'- caGGGUCUCCaGGUGCUuCUUCGCGU- -5'
miRNA: 3'- cAGGGUCUCCa-----------GGUGCUUCuuCGCGu -5'
miRNA: 3'- -CAGGGUCUCCAGGUGCUUCUUCGCGU- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14378 | 3' | -55.8 | NC_003521.1 | + | 239746 | 0.74 | 0.601003 |
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Target: 5'- -aUCCAGGGGUCgCGCGGcgAGAgcuGGCGCAg -3' miRNA: 3'- caGGGUCUCCAG-GUGCU--UCU---UCGCGU- -5' |
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| 14378 | 3' | -55.8 | NC_003521.1 | + | 185766 | 0.79 | 0.356493 |
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Target: 5'- -gCCUAGGGG-CCGCGAuGGAGCGCAa -3' miRNA: 3'- caGGGUCUCCaGGUGCUuCUUCGCGU- -5' |
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| 14378 | 3' | -55.8 | NC_003521.1 | + | 6361 | 0.8 | 0.325127 |
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Target: 5'- aUCCCAGAGGggccggucggggUCGCGGAGggGCGCc -3' miRNA: 3'- cAGGGUCUCCa-----------GGUGCUUCuuCGCGu -5' |
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| 14378 | 3' | -55.8 | NC_003521.1 | + | 56624 | 1.08 | 0.005079 |
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Target: 5'- cGUCCCAGAGGUCCACGAAGAAGCGCAc -3' miRNA: 3'- -CAGGGUCUCCAGGUGCUUCUUCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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