Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1438 | 3' | -58.1 | NC_001335.1 | + | 35297 | 0.7 | 0.331809 |
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Target: 5'- cGGCGGCuGGUGCGGGaaCAGCuccgggaacaccUCGGCCc -3' miRNA: 3'- -CCGCCG-CUACGUUUc-GUCG------------AGCCGGc -5' |
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| 1438 | 3' | -58.1 | NC_001335.1 | + | 1181 | 0.7 | 0.331809 |
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Target: 5'- aGGCGGgGGuggagcUGCAggGAGCAGCggaggCGGCa- -3' miRNA: 3'- -CCGCCgCU------ACGU--UUCGUCGa----GCCGgc -5' |
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| 1438 | 3' | -58.1 | NC_001335.1 | + | 31051 | 0.7 | 0.301066 |
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Target: 5'- -uCGGUGA-GCuucuuGCGGUUCGGCCGg -3' miRNA: 3'- ccGCCGCUaCGuuu--CGUCGAGCCGGC- -5' |
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| 1438 | 3' | -58.1 | NC_001335.1 | + | 17177 | 0.71 | 0.272568 |
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Target: 5'- aGGC-GCGGUGCu--GCAGCUCGcuCCGa -3' miRNA: 3'- -CCGcCGCUACGuuuCGUCGAGCc-GGC- -5' |
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| 1438 | 3' | -58.1 | NC_001335.1 | + | 23719 | 0.71 | 0.265791 |
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Target: 5'- aGGCGGCGGggguaaagGCAuGGCccuGGCUgaCGGCUGg -3' miRNA: 3'- -CCGCCGCUa-------CGUuUCG---UCGA--GCCGGC- -5' |
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| 1438 | 3' | -58.1 | NC_001335.1 | + | 24127 | 0.74 | 0.165925 |
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Target: 5'- aGCGGCGGUGCu-GGC-GCUcgCGGCCa -3' miRNA: 3'- cCGCCGCUACGuuUCGuCGA--GCCGGc -5' |
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| 1438 | 3' | -58.1 | NC_001335.1 | + | 32674 | 0.76 | 0.1298 |
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Target: 5'- cGGCGGCGGUGgcCAGcAGCAGuCUCGcGCCc -3' miRNA: 3'- -CCGCCGCUAC--GUU-UCGUC-GAGC-CGGc -5' |
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| 1438 | 3' | -58.1 | NC_001335.1 | + | 5550 | 0.76 | 0.126268 |
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Target: 5'- gGGCGGCGGUGCcGAGCAGgUagaUGGUCu -3' miRNA: 3'- -CCGCCGCUACGuUUCGUCgA---GCCGGc -5' |
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| 1438 | 3' | -58.1 | NC_001335.1 | + | 4098 | 1.1 | 0.000398 |
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Target: 5'- cGGCGGCGAUGCAAAGCAGCUCGGCCGc -3' miRNA: 3'- -CCGCCGCUACGUUUCGUCGAGCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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