Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1440 | 3' | -56 | NC_001335.1 | + | 26547 | 0.66 | 0.67841 |
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Target: 5'- cGCAUcaGCUCCucgaCCGAcucgcgcUCCACGCgGAu -3' miRNA: 3'- -CGUA--CGAGGug--GGCU-------AGGUGCGaCUc -5' |
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| 1440 | 3' | -56 | NC_001335.1 | + | 41106 | 0.66 | 0.646601 |
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Target: 5'- -gAUGUugUCCACgaCGAUCaACGCUGGGa -3' miRNA: 3'- cgUACG--AGGUGg-GCUAGgUGCGACUC- -5' |
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| 1440 | 3' | -56 | NC_001335.1 | + | 8822 | 0.67 | 0.602592 |
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Target: 5'- cGCGUcgagccGC-CCACCCGaAUgCACGCcGAGa -3' miRNA: 3'- -CGUA------CGaGGUGGGC-UAgGUGCGaCUC- -5' |
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| 1440 | 3' | -56 | NC_001335.1 | + | 20618 | 0.67 | 0.59163 |
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Target: 5'- ---cGCUCCA-CCGA-CCGCGCcGAGc -3' miRNA: 3'- cguaCGAGGUgGGCUaGGUGCGaCUC- -5' |
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| 1440 | 3' | -56 | NC_001335.1 | + | 52176 | 0.69 | 0.485171 |
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Target: 5'- aGCGUGCgauacaCGCCCauacCCGCGCUGAc -3' miRNA: 3'- -CGUACGag----GUGGGcua-GGUGCGACUc -5' |
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| 1440 | 3' | -56 | NC_001335.1 | + | 7169 | 0.69 | 0.464923 |
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Target: 5'- aCGUGUUCUucgUCUGGUCCugGCUGAc -3' miRNA: 3'- cGUACGAGGu--GGGCUAGGugCGACUc -5' |
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| 1440 | 3' | -56 | NC_001335.1 | + | 3238 | 0.69 | 0.464923 |
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Target: 5'- -gAUGCUCUGCCUGGUCaCAgGCcGGGa -3' miRNA: 3'- cgUACGAGGUGGGCUAG-GUgCGaCUC- -5' |
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| 1440 | 3' | -56 | NC_001335.1 | + | 32707 | 0.7 | 0.425804 |
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Target: 5'- cGCAgGCUCCGgUgGAUCCGCGCcGGc -3' miRNA: 3'- -CGUaCGAGGUgGgCUAGGUGCGaCUc -5' |
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| 1440 | 3' | -56 | NC_001335.1 | + | 5251 | 1.1 | 0.000721 |
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Target: 5'- gGCAUGCUCCACCCGAUCCACGCUGAGc -3' miRNA: 3'- -CGUACGAGGUGGGCUAGGUGCGACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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