Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1440 | 5' | -58.7 | NC_001335.1 | + | 41384 | 0.66 | 0.52339 |
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Target: 5'- gCGCGUAGGCGAggacgaacgccgacuUCcCGGUGccggGGCcgGCGc -3' miRNA: 3'- -GCGCGUCCGCU---------------AGaGCCAC----UCGa-CGU- -5' |
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| 1440 | 5' | -58.7 | NC_001335.1 | + | 43318 | 0.66 | 0.4884 |
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Target: 5'- uCGCGCGGucgucGUGGUCaaCGGUGAGCUuCGa -3' miRNA: 3'- -GCGCGUC-----CGCUAGa-GCCACUCGAcGU- -5' |
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| 1440 | 5' | -58.7 | NC_001335.1 | + | 51682 | 0.68 | 0.357971 |
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Target: 5'- aGCuGCAgauauGGCGcguUCUCGGUG-GCUGUAa -3' miRNA: 3'- gCG-CGU-----CCGCu--AGAGCCACuCGACGU- -5' |
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| 1440 | 5' | -58.7 | NC_001335.1 | + | 1164 | 0.77 | 0.098899 |
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Target: 5'- gGCGUAGGCGGcgUCggaggCGGgggugGAGCUGCAg -3' miRNA: 3'- gCGCGUCCGCU--AGa----GCCa----CUCGACGU- -5' |
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| 1440 | 5' | -58.7 | NC_001335.1 | + | 5286 | 1.08 | 0.000495 |
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Target: 5'- uCGCGCAGGCGAUCUCGGUGAGCUGCAc -3' miRNA: 3'- -GCGCGUCCGCUAGAGCCACUCGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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