Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14410 | 5' | -53.4 | NC_003521.1 | + | 134778 | 0.66 | 0.992709 |
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Target: 5'- uAGAGGGUCUugCCGAa--GuagUCGUAGg -3' miRNA: 3'- -UCUCCCAGA--GGCUgugCucaAGCAUC- -5' |
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| 14410 | 5' | -53.4 | NC_003521.1 | + | 102027 | 0.66 | 0.989272 |
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Target: 5'- uGGAGGGcaUCUCCuGgcgguugccGCACGAGguguagaggUCGUAGc -3' miRNA: 3'- -UCUCCC--AGAGG-C---------UGUGCUCa--------AGCAUC- -5' |
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| 14410 | 5' | -53.4 | NC_003521.1 | + | 83350 | 0.67 | 0.982867 |
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Target: 5'- -cAGGGg-UCCGuuGCaGAGUUCGUAGa -3' miRNA: 3'- ucUCCCagAGGCugUG-CUCAAGCAUC- -5' |
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| 14410 | 5' | -53.4 | NC_003521.1 | + | 223941 | 0.68 | 0.973929 |
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Target: 5'- gAGGGGGUCgaggCgGcCGCGGGgaacgcacgUCGUAGa -3' miRNA: 3'- -UCUCCCAGa---GgCuGUGCUCa--------AGCAUC- -5' |
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| 14410 | 5' | -53.4 | NC_003521.1 | + | 167764 | 0.7 | 0.927926 |
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Target: 5'- uGAGGGUCaCCGACAUGg---CGUGGu -3' miRNA: 3'- uCUCCCAGaGGCUGUGCucaaGCAUC- -5' |
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| 14410 | 5' | -53.4 | NC_003521.1 | + | 95899 | 0.7 | 0.916577 |
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Target: 5'- cGGGGaaugaucGUCUCCGAUGCGGGUU-GUGGu -3' miRNA: 3'- uCUCC-------CAGAGGCUGUGCUCAAgCAUC- -5' |
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| 14410 | 5' | -53.4 | NC_003521.1 | + | 100932 | 0.76 | 0.696898 |
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Target: 5'- --cGGGaUUUCCGcgGCACGGGUUCGUAGu -3' miRNA: 3'- ucuCCC-AGAGGC--UGUGCUCAAGCAUC- -5' |
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| 14410 | 5' | -53.4 | NC_003521.1 | + | 69569 | 1.09 | 0.008585 |
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Target: 5'- cAGAGGGUCUCCGACACGAGUUCGUAGa -3' miRNA: 3'- -UCUCCCAGAGGCUGUGCUCAAGCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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