Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1442 | 3' | -56.3 | NC_001335.1 | + | 666 | 0.66 | 0.659494 |
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Target: 5'- cUCGACCgCGuaaAGcAGGUCGgcGCUGCACUc -3' miRNA: 3'- uAGUUGG-GC---UC-UCCAGC--UGACGUGGc -5' |
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| 1442 | 3' | -56.3 | NC_001335.1 | + | 17904 | 0.66 | 0.647412 |
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Target: 5'- -cCAGCCCagcgagaaucccgGAGGGGUCGG-UGC-CCGa -3' miRNA: 3'- uaGUUGGG-------------CUCUCCAGCUgACGuGGC- -5' |
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| 1442 | 3' | -56.3 | NC_001335.1 | + | 51649 | 0.67 | 0.59357 |
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Target: 5'- --gGAUCCGAGAGGaUCGuGCcgGUGCCGg -3' miRNA: 3'- uagUUGGGCUCUCC-AGC-UGa-CGUGGC- -5' |
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| 1442 | 3' | -56.3 | NC_001335.1 | + | 3243 | 0.67 | 0.582643 |
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Target: 5'- uGUCuacCCCGAGGGGgagCGGCUGgUAgCGg -3' miRNA: 3'- -UAGuu-GGGCUCUCCa--GCUGAC-GUgGC- -5' |
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| 1442 | 3' | -56.3 | NC_001335.1 | + | 9211 | 0.69 | 0.466784 |
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Target: 5'- cAUCAGCUCG-GcGGUCGcCUGCAUCa -3' miRNA: 3'- -UAGUUGGGCuCuCCAGCuGACGUGGc -5' |
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| 1442 | 3' | -56.3 | NC_001335.1 | + | 5953 | 0.7 | 0.38148 |
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Target: 5'- --aGGCCCGAGcGGGUCGGgcuggcCUGUGCCGu -3' miRNA: 3'- uagUUGGGCUC-UCCAGCU------GACGUGGC- -5' |
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| 1442 | 3' | -56.3 | NC_001335.1 | + | 4674 | 0.79 | 0.114292 |
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Target: 5'- -aCAACCCGAGGGuGUCGACUGaCugCu -3' miRNA: 3'- uaGUUGGGCUCUC-CAGCUGAC-GugGc -5' |
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| 1442 | 3' | -56.3 | NC_001335.1 | + | 6129 | 1.07 | 0.000926 |
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Target: 5'- cAUCAACCCGAGAGGUCGACUGCACCGg -3' miRNA: 3'- -UAGUUGGGCUCUCCAGCUGACGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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