Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1443 | 3' | -56.8 | NC_001335.1 | + | 28024 | 0.66 | 0.621298 |
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Target: 5'- cGGcgUC-CCAGGUaugggaugaCGCUGgCC-CGGAa -3' miRNA: 3'- aCCuaAGuGGUCCA---------GCGAC-GGaGCCU- -5' |
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| 1443 | 3' | -56.8 | NC_001335.1 | + | 45307 | 0.66 | 0.610329 |
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Target: 5'- cGGGUUCGUCGGGcCGCUGUCggCGa- -3' miRNA: 3'- aCCUAAGUGGUCCaGCGACGGa-GCcu -5' |
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| 1443 | 3' | -56.8 | NC_001335.1 | + | 10895 | 0.69 | 0.442678 |
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Target: 5'- ---cUUCGCCAGGagGCUGCagccgCUCGGGu -3' miRNA: 3'- accuAAGUGGUCCagCGACG-----GAGCCU- -5' |
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| 1443 | 3' | -56.8 | NC_001335.1 | + | 24495 | 0.69 | 0.433014 |
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Target: 5'- gUGG--UCACCAGGaa--UGCCUCGGGg -3' miRNA: 3'- -ACCuaAGUGGUCCagcgACGGAGCCU- -5' |
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| 1443 | 3' | -56.8 | NC_001335.1 | + | 7289 | 0.69 | 0.414062 |
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Target: 5'- cGGGgcu-CgAGGUCGCUGCCUCa-- -3' miRNA: 3'- aCCUaaguGgUCCAGCGACGGAGccu -5' |
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| 1443 | 3' | -56.8 | NC_001335.1 | + | 6231 | 1.08 | 0.000688 |
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Target: 5'- gUGGAUUCACCAGGUCGCUGCCUCGGAc -3' miRNA: 3'- -ACCUAAGUGGUCCAGCGACGGAGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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