Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1444 | 3' | -51.3 | NC_001335.1 | + | 358 | 0.66 | 0.89849 |
Target: 5'- -aGAUGAGCGCcugguGGCGcucUUAUCGc -3' miRNA: 3'- caCUACUCGCGau---CCGCua-AGUAGCc -5' |
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1444 | 3' | -51.3 | NC_001335.1 | + | 1155 | 0.66 | 0.891179 |
Target: 5'- -gGAUcGGGCgGCgUAGGCGg--CGUCGGa -3' miRNA: 3'- caCUA-CUCG-CG-AUCCGCuaaGUAGCC- -5' |
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1444 | 3' | -51.3 | NC_001335.1 | + | 40685 | 0.67 | 0.86759 |
Target: 5'- -cGAUGAGUGCgguGGCGAUg-AUCu- -3' miRNA: 3'- caCUACUCGCGau-CCGCUAagUAGcc -5' |
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1444 | 3' | -51.3 | NC_001335.1 | + | 10761 | 0.74 | 0.465027 |
Target: 5'- aUGGUGAuuucgccgcGUGCUAGGCGg--CGUCGGa -3' miRNA: 3'- cACUACU---------CGCGAUCCGCuaaGUAGCC- -5' |
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1444 | 3' | -51.3 | NC_001335.1 | + | 6399 | 1.12 | 0.001519 |
Target: 5'- cGUGAUGAGCGCUAGGCGAUUCAUCGGa -3' miRNA: 3'- -CACUACUCGCGAUCCGCUAAGUAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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