Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14473 | 3' | -46.9 | NC_003521.1 | + | 103068 | 0.72 | 0.995549 |
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Target: 5'- gGCGgGUGCgcGGGCGAGGgcugcugcugGGGAUGCg -3' miRNA: 3'- -CGCaCACGaaCCUGCUCUa---------CUUUAUG- -5' |
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| 14473 | 3' | -46.9 | NC_003521.1 | + | 82780 | 0.72 | 0.993972 |
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Target: 5'- cCGUGgaGCacagGGACGAGGUGGAcgACg -3' miRNA: 3'- cGCACa-CGaa--CCUGCUCUACUUuaUG- -5' |
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| 14473 | 3' | -46.9 | NC_003521.1 | + | 99976 | 0.73 | 0.986424 |
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Target: 5'- aGCGUGUGCaggUUGGGCGuGuUGAAGacgGCg -3' miRNA: 3'- -CGCACACG---AACCUGCuCuACUUUa--UG- -5' |
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| 14473 | 3' | -46.9 | NC_003521.1 | + | 81596 | 0.74 | 0.980615 |
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Target: 5'- -gGUGgccgucauCUUGGACGAGGUGAAGUGa -3' miRNA: 3'- cgCACac------GAACCUGCUCUACUUUAUg -5' |
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| 14473 | 3' | -46.9 | NC_003521.1 | + | 135128 | 0.75 | 0.967089 |
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Target: 5'- gGUG-GUGUUggUGGACGAGAUGAAGa-- -3' miRNA: 3'- -CGCaCACGA--ACCUGCUCUACUUUaug -5' |
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| 14473 | 3' | -46.9 | NC_003521.1 | + | 122766 | 1.15 | 0.01635 |
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Target: 5'- aGCGUGUGCUUGGACGAGAUGAAAUACa -3' miRNA: 3'- -CGCACACGAACCUGCUCUACUUUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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