miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1448 5' -54.7 NC_001335.1 + 32902 0.66 0.67627
Target:  5'- cGGcucGGGCugGGCAGGGGcggUGCUc -3'
miRNA:   3'- aCCua-UUCGugCCGUCCCUcaaGCGA- -5'
1448 5' -54.7 NC_001335.1 + 42295 0.67 0.631541
Target:  5'- cGGAUucGAGCgugacgacGCGGCAGaGGAGcugcacgCGCUg -3'
miRNA:   3'- aCCUA--UUCG--------UGCCGUC-CCUCaa-----GCGA- -5'
1448 5' -54.7 NC_001335.1 + 7922 0.76 0.20647
Target:  5'- gGGAUccccagAAGUACGGCGGGGAGguucccCGCg -3'
miRNA:   3'- aCCUA------UUCGUGCCGUCCCUCaa----GCGa -5'
1448 5' -54.7 NC_001335.1 + 7780 1.07 0.001262
Target:  5'- cUGGAUAAGCACGGCAGGGAGUUCGCUc -3'
miRNA:   3'- -ACCUAUUCGUGCCGUCCCUCAAGCGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.