Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
145 | 3' | -63.7 | AC_000006.1 | + | 10657 | 0.66 | 0.230928 |
Target: 5'- --aCCGCGaCCGCGGCCguagcaGGCGccggCGCu -3' miRNA: 3'- guaGGCGC-GGCGUCGGg-----CCGCca--GUG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 10969 | 0.66 | 0.230928 |
Target: 5'- --gUCGCGCaGCAcGCgCUGGCGGUC-Ca -3' miRNA: 3'- guaGGCGCGgCGU-CG-GGCCGCCAGuG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 16564 | 0.66 | 0.230928 |
Target: 5'- --aCCggGUGCCGCgguAGCCCGGC-GUCGg -3' miRNA: 3'- guaGG--CGCGGCG---UCGGGCCGcCAGUg -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 6713 | 0.66 | 0.230928 |
Target: 5'- -uUCCGCGCCgaGCAGCCCcaGCG--CACc -3' miRNA: 3'- guAGGCGCGG--CGUCGGGc-CGCcaGUG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 19256 | 0.66 | 0.230336 |
Target: 5'- --gCCGCGUgGUAGCCCccucgcuGGUGGaCGCc -3' miRNA: 3'- guaGGCGCGgCGUCGGG-------CCGCCaGUG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 3680 | 0.66 | 0.219324 |
Target: 5'- --gCgGCuGCCGCGGCUgCGGCGGUg-- -3' miRNA: 3'- guaGgCG-CGGCGUCGG-GCCGCCAgug -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 3619 | 0.66 | 0.219324 |
Target: 5'- aCAgcaC-CGCCGCAGCCgCGGCaGcCGCa -3' miRNA: 3'- -GUag-GcGCGGCGUCGG-GCCGcCaGUG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 12120 | 0.66 | 0.219324 |
Target: 5'- --gCCGUGgCGCAGCgCGaGCGGuUCAa -3' miRNA: 3'- guaGGCGCgGCGUCGgGC-CGCC-AGUg -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 15876 | 0.66 | 0.21371 |
Target: 5'- ---gCGUGuuGCGGCCCGGCacGGcggugguguUCACg -3' miRNA: 3'- guagGCGCggCGUCGGGCCG--CC---------AGUG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 8284 | 0.66 | 0.202854 |
Target: 5'- --aCCGUGCCGCGGUgCGcuuuuagaaGCGGugUCGCg -3' miRNA: 3'- guaGGCGCGGCGUCGgGC---------CGCC--AGUG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 23227 | 0.67 | 0.190963 |
Target: 5'- --cCCGCGCCgacGCgcuccucgcucuggGGCCCGGCGcG-CGCa -3' miRNA: 3'- guaGGCGCGG---CG--------------UCGGGCCGC-CaGUG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 10972 | 0.67 | 0.17779 |
Target: 5'- --cCCGCGCCGC-GCacguggCGGCGGcCAa -3' miRNA: 3'- guaGGCGCGGCGuCGg-----GCCGCCaGUg -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 17429 | 0.67 | 0.177318 |
Target: 5'- gCAUCUG-GCCGCGGCCgcuucucgcccuuCGGCGG-CGg -3' miRNA: 3'- -GUAGGCgCGGCGUCGG-------------GCCGCCaGUg -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 17335 | 0.67 | 0.173119 |
Target: 5'- uUAUCuCGCGCUGCAcuGCCUGGU--UCGCg -3' miRNA: 3'- -GUAG-GCGCGGCGU--CGGGCCGccAGUG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 14263 | 0.69 | 0.139548 |
Target: 5'- aGUCCaCcCCGCAGCCCGGCaGcagCACc -3' miRNA: 3'- gUAGGcGcGGCGUCGGGCCGcCa--GUG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 14679 | 0.69 | 0.139548 |
Target: 5'- -cUCCGCGCCGCAggugacGUCCGG-GGUgGu -3' miRNA: 3'- guAGGCGCGGCGU------CGGGCCgCCAgUg -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 414 | 0.69 | 0.135797 |
Target: 5'- -uUCCGCGuCCGUgucaaAGUCCGGUGuuuauGUCACa -3' miRNA: 3'- guAGGCGC-GGCG-----UCGGGCCGC-----CAGUG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 15155 | 0.69 | 0.129989 |
Target: 5'- cCGUCCGCGuCCGCGGCCaCuuccgcgcuccguggGGCGcUUACa -3' miRNA: 3'- -GUAGGCGC-GGCGUCGG-G---------------CCGCcAGUG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 10713 | 0.72 | 0.071591 |
Target: 5'- --cCUGCuacgGCCGCGGUCgCGGUGGUCGCc -3' miRNA: 3'- guaGGCG----CGGCGUCGG-GCCGCCAGUG- -5' |
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145 | 3' | -63.7 | AC_000006.1 | + | 23682 | 0.74 | 0.050883 |
Target: 5'- --cUUGCGCCGCugcuccagGGCCUGGgGGUCGCg -3' miRNA: 3'- guaGGCGCGGCG--------UCGGGCCgCCAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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